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  • MABF543 - Anti-CD127 (IL-7Ra) Antibody (human), PE, clone A7R34

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MABF543 Sigma-Aldrich

Anti-CD127 (IL-7Ra) Antibody (human), PE, clone A7R34

clone R34-34, from mouse

Synonym: Interleukin-7 receptor subunit alpha, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, CDw127, CD antigen CD127

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, CD0-CDC, Primary Antibodies
conjugate   PE
clone   R34-34, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
species reactivity   human
shipped in   wet ice
packaging   pkg of 5 μL (0.5 μg/test)
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
UniProt accession no.   P16871
Gene Information   human ... IL7R(3575)

Description

General description

The R34-34 antibody is specific for human CD127, a cell surface protein also known as the Interleukin-7 Receptor alpha chain, or IL-7R alpha. CD127 is typically expressed at the cell surface as a heterodimer with the common gamma chain (CD132). This complex acts as the functional receptor for IL-7, a cytokine important in T and B cell development, and in mature T cell homeostasis. A second cytokine known as Thymic Stromal Lymphopoietin (TSLP) also binds to a receptor complex of CD127 and the TSLPR chain to trigger activation of dendritic cells, and is involved in B cell development, allergy and autoimmunity. The R34-34 antibody may be used as a phenotypic marker for CD127 on immature B cells, on subsets of thymocytes which are double negative (CD4-CD8- ) or single positive (CD4+ or CD8+), and at low levels on mature, peripheral T cells. CD127 is a key marker, when used in combination with CD4 and CD25, to distinguish Treg and effector/memory Treg populations known as T(REM), whereas certain populations of Foxp3+ /CD25+ T cells lacking CD127 have been associated with impaired suppressive function (Bikker et al. 2012. Rheumatology, 51: 996-1005)

Application

This mouse monoclonal antibody Anti-CD127 (IL-7Ra) (human), PE & clone A7R34 validated for use in Flow Cytometry for the detection of CD127 (IL-7Ra).

Quality

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 µg of this antibody detected CD127 (IL-7Ra) in one million human peripheral blood lymphocytes.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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