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  • MABF588 - Anti-CD45.1 Antibody (mouse), PE, clone A20

MABF588 Sigma-Aldrich

Anti-CD45.1 Antibody (mouse), PE, clone A20

This mouse monoclonal antibody Anti-CD45.1 (mouse), PE, clone A20 validated for use in IP, IHC & Flow Cytometry for the detection of CD45.1.

Synonym: Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45

  •  eCl@ss 32160702



Related Categories Antibodies, Primary Antibodies More...
packaging   25 µg (MABF588)
format   PE
application(s)   Flow Cytometry
isotype   IgG2aκ
clone   Monoclonal Antibody
concentration   0.2 mg/mL
host   Mouse
species reactivity   Mouse
gene symbol   Ly-5
UniProt accession no.   P06800
shipped in   1
Quality Assurance   The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 µg of this antibody detected CD45.1 in one million C57Bl/6 or SJL splenocytes.


General description

The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.

Safety & Documentation

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