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MABF590 Sigma-Aldrich

Anti-CD45.1 Antibody (mouse), APC-Cy7, clone A20

This mouse monoclonal antibody Anti-CD45.1 (mouse), APC-Cy7, clone A20 validated for use in IP, IHC & Flow Cytometry for the detection of CD45.1.

Synonym: Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45

  •  eCl@ss 32160702

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   25 µg (MABF590)
format   APC-Cy7
application(s)   Flow Cytometry
  Immunohistochemistry
  Immunoprecipitation
isotype   IgG2aκ
clone   Monoclonal Antibody
  A20
concentration   0.2 mg/mL
host   Mouse
species reactivity   Mouse
gene symbol   Ly-5
  Ptprc
UniProt accession no.   P06800
shipped in   1
Quality Assurance   The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 1 μg of this antibody detected CD45.1 in one million SJL splenocytes.

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Application

Research Category
Inflammation & Immunology

General description

The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.

Immunogen

Immunogen information for this antibody is proprietary information.

Physical form

Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Size Exclusion Chromatography

Storage and Stability

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
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