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  • MABF595 - Anti-CD45.2 Antibody (mouse), APC-Cy7, clone 104

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MABF595 Sigma-Aldrich

Anti-CD45.2 Antibody (mouse), APC-Cy7, clone 104

This mouse monoclonal antibody Anti-CD45.2 (mouse), APC-Cy7, clone 104 validated for use in IP, Flow Cytometry & ICC for the detection of CD45.2.

Synonym: Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45

  •  eCl@ss 32160702

  •  NACRES NA.44

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   25 µg (MABF595)
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
biological source   mouse
clone   104, monoclonal
  monoclonal
concentration   0.2 mg/mL
isotype   IgG2aκ
shipped in   wet ice
species reactivity   mouse
gene symbol   Ly-5
  Ptprc
conjugate   APC-Cy7

Description

General description

The 104 antibody reacts with mouse CD45.2, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The 104 antibody is typically used as a leukocyte marker in Ly5.2 mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. The antibody has been demonstrated to specific for CD45.2 and is not cross-reactive with CD45.1-bearing cells.

Quality

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 μg of this antibody detected CD45.2 in one million C57Bl/6 splenocytes.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles
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