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MABF610 Sigma-Aldrich

Anti-CD45RA Antibody (human), PerCP-Cy5.5, clone HI100

This mouse monoclonal antibody Anti-CD45RA (human), PerCP-Cy5.5, clone HI100 validated for use in western blotting, IHC & Flow Cytometry for the detection of CD45RA.

Synonym: Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD antigen CD45

  •  eCl@ss 32160702

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Properties

Related Categories Antibodies, Primary Antibodies More...
gene symbol   PTPRC(5788)
  CD45
UniProt accession no.   P08575
Quality Assurance   The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.06 µg of this antibody detected CD45RA in one million human peripheral blood lymphocytes.
application(s)   Flow Cytometry, Immunohistochemistry, Western Blotting
clone   HI100
  Monoclonal Antibody
concentration   5 µL (0.06 µg)/test
format   PerCP-Cy5.5
host   Mouse
isotype   IgG2bκ
packaging   25 tests (MABF610)
shipped in   wet ice
species reactivity   Human

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Application

Research Category
Inflammation & Immunology

General description

The HI100 antibody reacts with the human CD45 isoform known as CD45RA, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RB, CD45RO and others.The HI100 antibody is widely used as a marker for human CD45RA expression on naïve and activated T cells, B cells, and monocytes.

Immunogen

Immunogen information for this antibody is proprietary information.

Physical form

Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Size Exclusion Chromatography

Storage and Stability

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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