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MABN108 Sigma-Aldrich

Anti-Synaptotagmin 3 Antibody, clone 1E4.2

clone 1E4.2, from mouse

Synonym: Synaptotagmin-3, Synaptotagmin III, SytIII

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

clone   1E4.2, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   human, rat
shipped in   wet ice
isotype   IgG2bκ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_001153800
UniProt accession no.   Q9BQG1
Gene Information   human ... SYT3(84258)

Description

General description

Synaptotagmin 3 (SYT3; synaptogamin III) is a member of the synaptotagmin family, a class of transmembrane vesicular proteins with a well-documented role in calcium-dependent neurotransmitter release. Like other members of this family, synaptotagmin 3 contains a single transmembrane domain and C2 calcium-sensing domains which bind both calcium and phospholipids. The role of synaptotagmin 3 is not well understood; however, previous studies have indicated that SYT3 may play a role in insulin release in pancreatic β cells. STY3 may also play a role in CXCR4-mediated T-cell migration processes.

Immunogen

GST-tagged recombinant protein corresponding to human Synaptotagamin 3.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Synaptotagamin in human substantia nigra tissue.

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

This Anti-Synaptotagmin 3 Antibody, clone 1E4.2 is validated for use in Western Blotting, IHC for the detection of Synaptotagmin 3.

Target description

~70 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Synaptotagamin 3 in 10 µg of rat brain tissue lysate.

Analysis Note

Control
Rat brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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