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MABN109 Sigma-Aldrich

Anti-Synaptotagmin-4 Antibody, clone 10C11.1

clone 10C11.1, from mouse

Synonym: Synaptotagmin-4, Synaptotagmin IV, SytIV

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SU-SY
clone   10C11.1, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable (paraffin)
  western blot: suitable
species reactivity   human, human, mouse, mouse, mouse, human
shipped in   wet ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_065834
UniProt accession no.   Q9H2B2
Gene Information   human ... SYT4(6860)

Description

General description

Synaptotagmin-4 (UniProt Q9H2B2; also known as Synaptotagmin IV, SytIV) is encoded by the SYT4 (also known as KIAA1342) gene (Gene ID 6860) in human. The mammalian synaptotagmin family is comprised of 17 members. While most of them are predominantly function in the cetnral nervous system (CNS), some are involved in the vesicular functions of endocrine cells and glucose-transport metabolic cells. Synaptotagmin-4 is an inducible member detectable only in the brain and the neuroendocrine system. Unlike other family members, synaptotagmin-4 does not bind calcium and does not medidate calcium-induced exocytosis. Instead, synaptotagmin-4 is shown to play a neuroendocrine role in inhibiting exocytotic activities in the posterior pituitary and in cultured PC12 cells. Synaptotagmin-4 is expressed most abundantly in oxytocin neurons of the hypothalamus, where it negatively regulates oxytocin exocytosis. Dietary obesity is associated with increased vesicle binding of synaptotagmin-4 and decreased oxytocin release. Normalizing oxytocin release via Synaptotagmin-4 inhibition prevents against dietary obesity under chronic nutritional excess. Human synaptotagmin-4 consists of a vesiclular domain (a.a. 1-16), a transmembrane segment (a.a. 17-37),and a large cytoplasmic domain (a.a. 38-425).

Specificity

Clone 10C11.1 targets a cytoplasmic epitope present in both human spliced isoforms reported by UniProt (Q9H2B2).

Immunogen

Epitope: Cytoplasmic domain.

GST-tagged recombinant human Synaptotagmin-4 cytoplasmic domain fragment.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Synaptotagmin-4 in human kidney, skeletal muscle, and pancreas tissue sections.

Research Category
Neuroscience

Research Sub Category
Vesicular Trafficking

This Anti-Synaptotagmin-4 Antibody, clone 10C11.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Synaptotagmin-4.

Target description

~48 kDa observed. 47.96/46.06 kDa (human isoform 1/2) and 47.66 kDa (mouse) calculated.

Physical form

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in mouse cerebellum tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Synaptotagmin-4 in 10 µg of mouse cerebellum tissue lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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