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MABN1101 Sigma-Aldrich

Anti-SRY, clone 1G4 Antibody

Anti-SRY, clone 1G4 Antibody is an antibody against SRY for use in western blotting, IHC, flow cytometry & ELISA.

Synonym: Sex-determining region Y protein, Testis-determining factor, SRY

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 µL (MABN1101)
application(s)   ELISA: suitable
  flow cytometry: suitable
  immunohistochemistry: suitable
  western blot: suitable
biological source   mouse
clone   1G4, monoclonal
isotype   IgG1
shipped in   wet ice
species reactivity   human
gene symbol   SRY(6736)
format   Purified
molecular weight   ~36 kDa observed. Uncharacterized bands may be observed in some lysate(s).
clone   Monoclonal Antibody
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
gene symbol   TDF
Quality Assurance   Evaluated by Western Blotting in NTERA-2 cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected SRY in NTERA-2 cell lysate.

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

~36 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Application

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected SRY in cervical and ovarian cancer tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected SRY in HepG2 cells.

ELISA: A representative lot detected control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

General description

Sex-determining region Y protein, also known as Testis-determining factor, and encoded by the human gene SRY or TDF is a transcriptional regulator that controls a genetic switch in male development. SRY is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) to Sertoli rather than granulosa cells. In male adult brain SRY is involved in the maintenance of motor functions of dopaminergic neurons. SRY is also involved in different aspects of gene regulation including promoter activation or repression, DNA bending and manipulation. The SRY HMG box recognizes DNA by partial intercalation into the minor groove, and SRY is also involved in pre-mRNA splicing. SRY is expressed in the developing testis and surrounding tissue and can be found expressed in neurons in the adult. SRY is nuclear associated.

Immunogen

Purified recombinant fragment of human SRY expressed in E. Coli

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal in PBS containing up to 0.1% sodium azide and 0.5% protein stabilizer.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Making Headway
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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