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MABN1122 Sigma-Aldrich

Anti-PSMA7, clone EPR5836, Rabbit Monoclonal Antibody

clone EPR5836, from rabbit

Synonym: Proteasome subunit alpha type-7, Proteasome subunit RC6-1, Proteasome subunit XAPC7

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, PS-PS, Primary Antibodies
clone   EPR5836, monoclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG
Quality Level   300
antibody product type   primary antibodies
UniProt accession no.   O14818

Description

General description

PSMA (prostate specific membrane antigen) is a type II, integral membrane glycoprotein, composed of a 19 amino acid intracellular domain containing the N-terminus, a 24 amino acid transmembrane region, and a 707 amino acid extracellular C-terminal domain. The PSMA gene has been cloned and sequenced, and has been localized to chromosome 11q. PSMA is highly expressed in prostate secretory-acinar epithelium, in some benign extraprostatic epithelial cells from breast, duodenum, and kidney tissues, and in prostate cancer. Recently, evidence of limited and specific endothelial PSMA expression has been discovered: the neovasculature of a wide variety of malignant neoplasms (lung, colon, breast, etc.) demonstrates PSMA expression. This finding suggests that PSMA may be an effective target for monoclonal antibody-based anti-neovasculature therapy. PSMA has also been identified in the human nervous system. Based on activity, immunoreactivity, and mRNA sequence comparison with one form of NAALADase, a neuropeptide that may modulate glutaminergic transmission in the nervous system, PSMA was found to be indistinguishable from NAALADase derived from human cerebellar cell isolates. Therefore, the form of NAALADase also known as PSMA is expressed in brain, where it makes up a significant fraction of brain NAALADase activity.

Immunogen

Synthetic peptide corresponding to human PSMA7.

Application

Immunohistochemistry Analysis: A 1:250-500 dilution from a representative lot detected PSMA7 in human breast carcinoma tissue.

Produced in collaboration with Epitomics

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

This Anti-PSMA7, clone EPR5836, Rabbit Monoclonal Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of PSMA7.

Target description

~27 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Unpurified

Rabbit monoclonal in buffer containing PBS with up to 0.1% sodium azide, 0.05% BSA, and 50% glycerol.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in A549, MCF-7, HEK293, and HeLa cell lysates.

Western Blotting Analysis: A 1:1,000-10,000 dilution of this antibody detected PSMA7 in A549, MCF-7, HEK293, and HeLa cell lysates.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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