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  • MABN1802 - Anti-pan-PMCA Antibody, clone 5F10

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MABN1802 Sigma-Aldrich

Anti-pan-PMCA Antibody, clone 5F10

clone 5F10, from mouse

Synonym: Plasma membrane calcium-transporting ATPase, Plasma membrane calcium pump

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, P-PA, Primary Antibodies
clone   5F10, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable (paraffin)
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human, chicken, rat, chicken, human, rat
shipped in   ambient
packaging   antibody small pack of 25 μg
isotype   IgG2aκ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_001001323.1
  NP_001001331
  NP_001001396.1
  NP_068768.2
UniProt accession no.   P20020
  P23634
  Q01814
  Q16720
Gene Information   human ... ATP2B1(490), ATP2B3(492), ATP2B4(493)

Description

General description

Plasma membrane calcium-transporting ATPase (isoforms 1-4) UniProt: P20020, Q01814, Q16720, P23634; also known as PMCA, Plasma membrane calcium pump) is encoded by the ATP2B1, ATP2B2, ATP2B3, ATP2B4 genes (Gene ID: 490; 491; 492; 493) in human. PMCAs are multi-pass membrane proteins that act as a calcium/calmodulin-regulated and magnesium-dependent enzymes and catalyze the hydrolysis of ATP coupled with the transport of calcium out of the cell. PMCAs serve as the main off switch for calcium signals in all types of cells. Although there are four separate genes that code for PMCAs 1-4, alternative splicing of the primary transcripts further increases the number of PMCA variants to about 20, which differ significantly in their regulatory and kinetic properties. PMCA activity is shown to be regulated at (a) the transcriptional level (b) by calmodulin binding and (c) by their subcellular localization. The localization of different PMCAs is regulated in various complex, cell type-specific ways. PMCA1 is ubiquitously expressed, PMCA2 is mainly expressed in brain cortex, but lower levels are also found in skeletal muscle, heart muscle, stomach, liver, kidney and lung. PMCA3 is highly expressed in the cerebellum, particularly in the presynaptic terminals of parallel fibers-Purkinje neurons. PMCA4 is expressed ubiquitously, however, its tissue distribution varies with its isoforms. (Ref.: Antalffy, G et al. (2013). Biochim. Biophys. Acta 1833(12); 2561-2572; Strehler, EE (2015). Biochem. Biophys. Commun. 460 (1); 26-33)

Specificity

Clone 5F10 detects all isoforms of Plasma membrane calcium-transporting ATPase (PMCA) in human, mouse, and rat.

Immunogen

Purified human erythrocyte calcium ATPase.

Application

Anti-pan-PMCA, clone 5F10, Cat. No. MABN1802, is a mouse monoclonal antibody that targets pan-Plasma membrane calcium-transporting ATPase (PMCA) and has been tested in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected pan-PMCA in human pancreas and human kidney tissue.

Immunocytochemistry Analysis: A representative lot detected pan-PMCA in Immunocytochemistry applications (Paszty, K., et. al. (2005). Biochem J. 391(Pt 3):687-92; DeMarco, S.J., et. al. (2002) J Biol Chem. 277(12):10506-11).

Immunoprecipitation Analysis: A representative lot immunoprecipitated pan-PMCA in Immunoprecipitation applications (DeMarco, S.J., et. al. (2002) J Biol Chem. 277(12):10506-11).

Western Blotting Analysis: A representative lot detected pan-PMCA in Western Blotting applications (Paszty, K., et. al. (2005). Biochem J. 391(Pt 3):687-92; Caride, A.J., et. al. (1996). Biochem J. 316 ( Pt 1):353-9).

ELISA Analysis: A representative lot detected pan-PMCA in ELISA applications (Caride, A.J., et. al. (1996). Biochem J. 316 ( Pt 1):353-9).

Research Category
Neuroscience

Target description

134 to 139 kDa calculated.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Immunohistochemistry (Paraffin) in human cerebral cortex tissue

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected pan-PMCA in human cerebral cortex tissue sections.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Flash Point(F) 
does not flash
Flash Point(C) 
does not flash
Protocols & Articles

Articles

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Protocols

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