• USA Home
  • MABN250 - Anti-RA-induced protein 17 Antibody, clone 5H5.1

EMAIL THIS PAGE TO A FRIEND
MABN250 Sigma-Aldrich

Anti-RA-induced protein 17 Antibody, clone 5H5.1

clone 5H5.1, from mouse

Synonym: Retinoic acid-induced protein 17, ZMIZ1, Zinc finger MIZ domain-containing protein 1, PIAS-like protein Zimp10

  •  eCl@ss 32160702

  •  NACRES NA.41

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, RAE-RAX
clone   5H5.1, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_065071
UniProt accession no.   Q9ULJ6
Gene Information   human ... ZMIZ1(57178)

Description

General description

Retinoic acid-induced protein 17 (RAI17) is also called Zinc finger MIZ domain-containing protein 1 (ZMIZ1), and PIAS-like protein Zimp10. Retinoic acid-induced protein 17 promotes AR sumoylation and increases AR ligand-dependent transcriptional activity. Retinoic acid-induced protein 17 is highly expressed in ovary and at lower levels in prostate, spleen and testis.

Immunogen

Recombinant protein corresponding to human RA-induced protein 17.

Application

Detect Retinoic acid-induced protein 17 using this mouse monoclonal antibody, Anti-RA-induced protein 17 Antibody, clone 5H5.1 validated for use in western blotting & IHC.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected RA-induced protein 17 in human thalamus tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Target description

~115 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing at ~115 kDa and ~107 kDa

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected RA-induced protein 17 in 10 µg of SH-SY5Y cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?