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  • MABN300 - Anti-Peroxiredoxin-5 (PRDX5) Human Antibody, clone 5 286 6F7

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MABN300 Sigma-Aldrich

Anti-Peroxiredoxin-5 (PRDX5) Human Antibody, clone 5 286 6F7

clone 5 286 6F7, from mouse

Synonym: Peroxiredoxin-5, mitochondrial, Alu corepressor 1, Antioxidant enzyme B166, AOEB166, Liver tissue 2D-page spot 71B, PLP, Peroxiredoxin V, Prx-V, Peroxisomal antioxidant enzyme, TPx type VI, Thioredoxin peroxidase PMP20, Thioredoxin reductase

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

clone   5 286 6F7, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_036226
UniProt accession no.   P30044
Gene Information   human ... PRDX5(25824)

Description

General description

Peroxiredoxin-5 (PRDX5) is a novel mammalian peroxiredoxin, belonging to the family of thiol-dependent peroxidases. It is structurally dissimilar from other peroxiredoxins in that it contains a conserved cysteine 47 residue at the N-terminus and a cysteine 151 residue at the C-terminus which form an intramolecular disulphide bridge and confers unique chemical characteristics to this enzyme. Peroxiredoxins protect cells from apoptosis induced by overexposure to endogenous or exogenous peroxides, such as peroxynitrites, hydrogen peroxide, and alkyl hydroperoxides. Peroxiredoxin-5 is widely expressed in various subcellular regions, including mitochondria, peroxisomes, nucleus, and cytosol.

Specificity

This protein does not react with Rat, Mouse, or Canine.

Immunogen

Recombinant protein corresponding to human Peroxiredoxin-5.

Application

Anti-Peroxiredoxin-5 (PRDX5) Human Antibody, clone 5 286 6F7 detects level of Peroxiredoxin-5 (PRDX5) Human & has been published & validated for use in Western Blotting & IHC.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Peroxiredoxin-5 in human chorionic villi, human placenta, and human adrenal gland tissues.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Target description

~17 kDa observed. An uncharacterized band may appear at ~53 kDa in some lysates.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot in human brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Peroxiredoxin-5 in 10 µg of human brain tissue lysate.

Analysis Note

Control
Human brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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