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MABN390 Sigma-Aldrich

Anti-Sigma IR Antibody, clone ICR-SIG1R-A

Detect the Sigma IR protein using this Anti-Sigma IR, clone ICR-SIG1R-A validated for use in wb, IP, ICC, Flow & IHC.

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SG-SK
clone   ICR-SIG1R-A, monoclonal
biological source   rat
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   mouse, human, human
species reactivity (predicted by homology)   mouse (immunogen homology)
shipped in   wet ice
isotype   IgG2c
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_005857
UniProt accession no.   Q99720
Gene Information   human ... SIGMAR1(10280)

Description

General description

Sigma1R, or Sigma non-opioid intracellular receptor 1, is part of the ERG2 family that forms a ternary complex with ANK2 and ITPR3. Sigma1R is involved in lipid transport from the endoplasmic reticulum, BDNF and EGF signaling, potassium ion channel regulation and other cell functions including apoptosis, survival and proliferation. It was initially discovered through its binding to various psychoactive drugs, and has been studied in memory, learning and mood alteration processes. Sigma1R is often found in the nuclear membrane, synapses and endoplasmic reticulum of cells within liver, brain, kidney and thymus tissues.

Immunogen

Linear peptide corresponding to human Sigma IR.

Application

Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to detect Sigma IR in certain cancer cell lysates.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to detect Sigma IR in human cancer cells including pancreatic carcinoma and sarcoma cell lines and xenografts.
Flow Cytometry Analysis: A representative lot was used by an an independent laboratory to detect Sigma IR in tumor cells.
Immunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect Sigma IR in human cancer cells including pancreatic carcinoma and sarcoma cell lines and xenografts.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Target description

~25 kDa observed. Uniprot describes 5 isoforms produced by alternative splicing at ~25 kDa, ~23 kDa, ~21.5 kDa, ~12 kDa and ~21 kDa

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in colo-587 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Sigma IR in 10 µg of colo-587 cell lysate.

Analysis Note

Control
Colo-587 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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