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MABN497 Sigma-Aldrich

Anti-PSD93 Antibody, clone N18/30

clone N18/30, from mouse

Synonym: Disks large homolog 2, Channel-associated protein of synapse-110, Chapsyn-110, Postsynaptic density protein PSD-93

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, PS-PS, Primary Antibodies
clone   N18/30, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   rat, rat, human, mouse, human, mouse
shipped in   wet ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_071618
UniProt accession no.   Q63622

Description

General description

As a member of the membrane-associated guanylate kinase (MAGUK) family, PSD93 (also known as Chapsyn-110) binds directly to the N-methyl-D-aspartate (NMDA) receptor and the Shaker K+ channel subunits. It shares 70-80% homology with PSD-95 (SAP90), and SAP97. Like PSD-95, PSD93 plays a key role in postsynaptic targeting. Aside from binding to glutamate receptors, the MAGUK family of proteins also interacts with cell surface structures, like cell adhesion molecules, ion channels, cytoskeletal elements, and signal transduction molecules. Improper PSD93 signaling has been implicated in certain disease states, such as schizophrenia.

Specificity

This antibody does not demonstrate cross reactivity with other MAGUK family members, such as PSD-95, SAP97, and SAP102 expressed in transfected cells.

Immunogen

Recombinant protein corresponding to rat PSD93.

Application

Detect PSD93 using this Anti-PSD93 Antibody, clone N18/30 validated for use in Western Blotting & IHC.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Western Blot Analysis: 1 µg/mL from a representative lot detected PSD93 in 10 µg of human brain tissue lysate.

Immunohistochemistry Analysis: A representative lot detected PSD93 in rat brain tissue (Prof. J. Trimmer, University of California, Davis).

Western Blot Analyis: A representative lot detected PSD93 in WT, but not in PSD93 knockout mice.

Western Blot Analysis: A representative lot detected PSD93 in CA1 tissue lysates from C57BL/6 mice (Jeffrey, R. A., et al. (2009). J Neurosci. 29(50):15613-15620.).

Target description

~110 kDa observed. The calculated molecular weight of PSD93 is 95 kDa; however, this protein is highly phosphorylated and is observed at ~110 kDa Multiple isoforms between 68-98 kDa are known to exist.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PSD93 in 10 µg of mouse brain tissue lysate.

Analysis Note

Control
Mouse brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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