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  • MABN50 - Anti-Olig2 Antibody, clone 211F1.1

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MABN50 Sigma-Aldrich

Anti-Olig2 Antibody, clone 211F1.1

clone 211F1.1, from mouse

Synonym: oligodendrocyte lineage transcription factor 2, oligodendrocyte-specific bHLH transcription factor 2, human protein kinase C-binding protein RACK17, Protein kinase C-binding protein 2, protein kinase C binding protein 2, Class B basic helix-loop-helix pr

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Antibodies for Neurobiology, Antibodies to Neural Proteins, Neuroscience, Primary Antibodies More...
clone   211F1.1, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   mouse, rat, mouse, human, mouse
species reactivity (predicted by homology)   human (based on 100% sequence homology), human (based on 100% sequence homology), rat (based on 100% sequence homology), rat (based on 100% sequence homology)
shipped in   wet ice
isotype   IgG2aκ
Quality Level   100
NCBI accession no.   NP_005797
UniProt accession no.   Q13516
Gene Information   human ... OLIG2(10215)

Description

General description

Oligodendrocyte lineage transcription factor 2 (Olig2) is a protein belonging to group A of the OLIG family of basic helix-loop-helix transcription factors (bHLH). bHLH proteins are divided into 6 groups (A-F), and characterized by their biochemical properties and structure. Olig2 is classified as group A due to its ability to form heterodimers with other bHLH proteins and binding to the E box. Olig2 has a crucial role during development in specifying the final location of motor neurons and oligodendrocytes in the spinal cord, along with the development within the hindbrain of somatic motor neurons. Olig2 expression is observed within oligodendrocytes and in developing astrocytes. Recent studies have shown strong expression of Olig2 in human oligodendrogliomas, primary tumors within the central nervous system, and moderate expression in astrocytomas. These observations have led to the proposal of Olig2 having potential as an oligodendroglioma diagnostic marker.

Specificity

This antibody recognizes Olig2.

Immunogen

Epitope: Unknown

Recombinant protein corresponding to human Olig2.

Application

Detect Olig2 using this Anti-Olig2 Antibody, clone 211F1.1 validated for use in WB, IC, IH & IP.

Immunohistochemistry Analysis: This antibody has been shown to detect Olig2 in coronal brain tissue as reported by an independent laboratory (Cai, J., et al. (2007). Development. 134:1887-1899).

Immunocytochemistry Analysis: This antibody has been shown to detect Olig2, as reported by an independent laboratory (Lee, S.K., et al. (1995). Genes & Dev. 19:282-294).

Immunoprecipitation Analysis: This antibody has been shown to detect Olig2, as reported by an independent laboratory (Lee, S.K., et al. (1995). Genes & Dev. 19:282-294).

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Target description

~37 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot in mouse brain lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected Olig2 on 10 µg of mouse brain lysate.

Analysis Note

Control
Mouse brain lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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