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MABN514 Sigma-Aldrich

Anti-Uncx Antibody, clone N155/9

clone N155/9, from mouse

Synonym: Homeobox protein unc-4 homolog, Homeobox protein Uncx4.1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, UH-UZ
clone   N155/9, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   mouse
shipped in   wet ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_038730
UniProt accession no.   O08934

Description

General description

Homeobox protein unc-4 homolog (Uncx) is also called homeobox protein Uncx4.1 and is expressed in paraxial mesoderm, the developing kidney and central nervous system. Uncx is a transcription factor involved in somitogenesis and neurogenesis. Uncx is important for the maintenance and differentiation of certain axial skeleton elements, and helps control the connections of hypothalamic neurons to pituitary elements, allowing central neurons to reach the peripheral blood circulation and to deliver hormones for control of peripheral functions.

Immunogen

Recombinant protein corresponding to mouse Uncx.

Application

Anti-Uncx Antibody, clone N155/9 is a highly specific mouse monoclonal antibody, that targets Uncx & has been tested in western blotting & IHC.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Uncx in 10 µg of mouse brain tissue lysate.
Immunofluorescence Analysis: A representative lot detected Uncx in mouse embryo cell lysate (Prof. J Trimmer, Univestiy of California, Davis).

Target description

~ 65 kDa observed. The calculated molecular weight of this protein is 54 kDa, but can be observed at ~65 kDa due to tissue lysates.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in mouse E16 brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Uncx in 10 µg of mouse E16 brain tissue lysate.

Analysis Note

Control
Mouse E16 brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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