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  • MABN59 - Anti-ACE2 Antibody, clone 4G5.1

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MABN59 Sigma-Aldrich

Anti-ACE2 Antibody, clone 4G5.1

Anti-ACE2 Antibody, clone 4G5.1 detects level of ACE2 & has been validated for use in Western Blotting.

Synonym: Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Primary Antibodies
clone   4G5.1, monoclonal
biological source   mouse
application(s)   western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG1κ
antibody product type   primary antibodies
NCBI accession no.   NP_068576
UniProt accession no.   Q9BYF1
Gene Information   human ... ACE2(59272)

Description

General description

ACE2 (Angiotensin-converting enzyme 2) is a transmembrane protein belonging to the peptidase M2 family. It is expressed in vascular endothelial cells of the heart, kidney, testis, and gastrointestinal system. ACE2 produces Angiotensin (1-9) by hydrolysis of Angiotensin 1. Angiotensin (1-9) is a precursor for Angiotensin (1-7). ACE2 may also directly produce Angiotensin (1-7) by conversion of Angiotensin II, which is involved in vasoconstriction. Angiotensin (1-7) plays an important role in the RAS signaling pathway and is involved in vasodilation. Hence, ACE2 antagonizes the effects of its homolog, ACE. ACE2 is insensitive to commonly used inhibitors such as linosipril and captopril. ACE2 is upregulated in heart failure, possibly in response to overactivity of Angiotensin II; ACE2 is studied for its potential therapeutic value for hypertension and heart failure.

Specificity

This antibody recognizes ACE2.

Immunogen

GST-tagged recombinant protein corresponding to human ACE2.

Application

ELISA Analysis: A representative lot detected ACE2 in a direct ELISA.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Target description

~62 kDa observed. Uniport describes 2 isoforms at ~92 kDa (Isoform 1) and ~64 kDa (Isoform 2) due to alternative splicing.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected ACE2 in 10 µg of HEK293 cell lysate.

Linkage

Replaces: MAB5676

Analysis Note

Control
HEK293 cell lysate

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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