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MABN674 Sigma-Aldrich

Anti-PSIP1 Antibody, clone 6E4

ascites fluid, clone 6E4, from mouse

Synonym: PSIP1 protein

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, PS-PS, Primary Antibodies
clone   6E4, monoclonal
biological source   mouse
application(s)   immunofluorescence: suitable
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   human, rat, monkey
shipped in   wet ice
antibody form   ascites fluid
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
UniProt accession no.   Q05CM9
Gene Information   human ... PSIP1(11168)

Description

General description

PSIP1, also known as coactivator p75, as well as numerous other synonyms named after their original discoveries including, PC4 and SFRS1 interacting protein 1, lens epithelium-derived growth factor (LEDGF/p75), dense fine speckles 70kD protein (DFS 70), and transcriptional coactivator p75/p52, is a transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. It is particularly important in lens epithelial cell gene regulation and stress responses, and even though initially thought to be limited to the lens of the eye, PSIP1 is now known to be widely expressed in most tissues and has no direct role in the development of lens epithelium. It also gained research interest as the focal point for HIV integration and appears to be one of the few integrase interactor proteins whose knockdown limits HIV integration and propagation. Moreover, PSIP1 is an anti-apoptotic protein and appears to play a protective role during stress-induced apoptosis. There are three isoforms of the PSIP1 in humans, PSIP1, PSIP2 or p52, and a third isoform which is unnamed. The PSIP1 isoforms seem to vary in their precise functions somewhat with isoform 2 being the most commonly expressed and most abundant. Expression is localized to the nucleus. EMD-Millipore’s anti-PSIP1mouse monoclonal, clone 6.00E+04 has been tested on human, paraffin embedded immunohistochemical sections, immunocytochemical immunofluorescence studies using mouse NIH3T3 cells, western blot using human, mouse, and rat cells line lysates, and ELISA using purified antigen.

Immunogen

Purified recombinant fragment of human PSIP1 expressed in E. Coli.

Application

Detect PSIP1 using this mouse monoclonal antibody, Anti-PSIP1 Antibody, clone 6E4 validated for use in western blotting, IHC & Immunofluorescence.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected PSIP1 in NIH/3T3 cells.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected PSIP1 in rat cerebellum, rat hippocampus, and human cerebellum tissues.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Target description

~75 kDa observed. Uncharacterized bands may appear in some lysate(s).

Physical form

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in HepG2, Jurkat, K562, Cos7, PC-12, HeLa, and NIH/3T3 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PSIP1 in HepG2, Jurkat, K562, Cos7, PC-12, HeLa, and NIH/3T3 cell lysates.

Analysis Note

Control
HepG2, Jurkat, K562, Cos7, PC-12, HeLa, and NIH/3T3 cell lysates

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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