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MABN68 Sigma-Aldrich

Anti-PSD95 Antibody, clone K28/43

clone K28/43, from mouse

Synonym: Postsynaptic density protein 95, Synapse-associated protein 90, Tax interaction protein 15, discs large homolog 4, discs, large homolog 4 (Drosophila), post-synaptic density protein 95

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, PS-PS, Primary Antibodies
clone   K28/43, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   rat, human, mouse, rat
species reactivity (predicted by homology)   human (immunogen homology), mouse (based on 100% sequence homology)
shipped in   wet ice
isotype   IgG2aκ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_001356
UniProt accession no.   P78352

Description

General description

PSD95 (postsynaptic density 95) represent a class of anchoring proteins which serve to localize various neuronal ion channels to post-synaptic densities. PSD95 and related proteins contain PDZ domains, which interact with NMDA receptors and voltage-gated K+ channels via a ET/SXV motif in the cytoplasmic carboxyl terminus of the receptor channels.

Immunogen

Recombinant protein corresponding to human PSD95.

Application

Detect PSD95 using this Anti-PSD95 Antibody, clone K28/43 validated for use in IH, WB & IC.

Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected PSD95 in rat cortex tissue.

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (Maeda, T., et al. (2005). Invest. Ophthalmol. Vis. Sci. 46(11):4320-4327.)

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Target description

~95 kDa observed.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected PSD95 on 10 µg of rat brain tissue lysate.

Linkage

Replaces: 04-1066

Analysis Note

Control
Rat brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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