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  • MABN790 - Anti-Shank3 Antibody, clone 16E2.3

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MABN790 Sigma-Aldrich

Anti-Shank3 Antibody, clone 16E2.3

This Anti-Shank3 Antibody, clone 16E2.3 is validated for use in Western Blotting for the detection of Shank3.

Synonym: SH3 and multiple ankyrin repeat domains protein 3, Shank3, Proline-rich synapse-associated protein 2, ProSAP2, Shank3

  •  eCl@ss 32160702

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SG-SK
clone   16E2.3, monoclonal
biological source   mouse
application(s)   western blot: suitable
species reactivity   mouse, mouse, mouse
species reactivity (predicted by homology)   human (based on 100% sequence homology), human (based on 100% sequence homology)
shipped in   wet ice
isotype   IgG2aκ
antibody product type   primary antibodies
NCBI accession no.   NP_277052
UniProt accession no.   Q9BYB0
Gene Information   human ... SHANK3(85358)

Description

General description

SH3 and multiple ankyrin repeat domains protein 3 (UniProt Q9BYB0; also known as Proline rich synapse associated protein 2, ProSAP2, Shank postsynaptic density protein, Shank3) is encoded by the SHANK3 (also known as DEL22q13.3, PROSAP2, PSAP2, SCZD15, SPANK-2) gene (Gene ID 85358) in human. Shank3/ProSAP2 belongs to the ProSAP/Shank family of scaffolding proteins, which are important dendritic spines postsynaptic density (PSD) complex components that play an important role in linking membrane glutamate receptors to intracellular signaling proteins and actin cytoskeleton. Shank proteins interact with adaptor proteins and assembles glutamate receptors at the postsynaptic membrane via multiple domains, including ankyrin repeat, proline-rich, SH3, PSD95/disc-large/zona occludens-1 (PDZ), and SAM domains. Shanks, together with the adaptor protein Homer and the endocytic vesicle-associated protein dynamin-3, are required for proper localization of the endocytic zone in close proximity to the PSD. Shank-3 induces formation of functional dendritic spines in aspiny cerebellar neurons, whereas Shank-3 knockdown results in reduced spine density and size in cultured hippocampal neurons. SHANK3 gene mutations are known causes of mental retardation and autism spectrum disorders.

Specificity

Expected to react with both isoform 1 (A) and 2 (B).

Immunogen

Epitope: Internal (C-terminal half)

GST-tagged recombinant protein corresponding to the C-terminal half of human Shank3.

Application

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Target description

~185 kDa observed. Multiplet banding pattern as a result of isoforms and/or posttranslational modifications. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Shank3 in 10 µg of mouse brain tissue lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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