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  • MABN863 - Anti-Cav1.2 alpha1c, clone 8F9.1 Antibody

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MABN863 Sigma-Aldrich

Anti-Cav1.2 alpha1c, clone 8F9.1 Antibody

clone 8F9.1, from mouse

Synonym: Voltage-dependent L-type calcium channel subunit alpha-1C, Calcium channel, L type. alpha-1 polypeptide, isoform 1, cardiac muscle, Voltage-gated calcium channel subunit alpha Cav1.2

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, CAM-CB, Primary Antibodies
clone   8F9.1, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable (paraffin)
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_000710
UniProt accession no.   Q13936
Gene Information   human ... CACNA1C(775)

Description

General description

This gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Many alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Immunogen

Recombinant protein corresponding to human Cav1.2 alpha1c.

Application

Anti-Cav1.2 alpha1c, clone 8F9.1 | MABN863 is an antibody against Cav1.2 alpha1c for use in Western Blotting, Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected Cav1.2 alpha1c in human cerebellum, human cerebral cortex, and human pancreas tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Target description

~230 kDa observed. UniProt entry Q13936 describes 37 isoforms produced by alternative splicing.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 5.0 µg/mL of this antibody detected Cav1.2 alpha1c in 10 µg of human brain tissue lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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