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  • MABS1304 - Anti-ATP Synthase subunit β Antibody, clone 11/21-7-A8

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MABS1304 Sigma-Aldrich

Anti-ATP Synthase subunit β Antibody, clone 11/21-7-A8

clone 11/21-7-A8, from mouse

Synonym: ATP synthase subunit beta, mitochondrial, ATP Synthase subunit β, beta-F1-ATPase

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories ATH-AZ, Alphabetical Index, Antibodies, Primary Antibodies
clone   11/21-7-A8, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  dot blot: suitable
  immunocytochemistry: suitable
  western blot: suitable
species reactivity   human, rat, mouse
shipped in   wet ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_001677
UniProt accession no.   P06576
Gene Information   human ... ATP5B(506)

Description

General description

ATP synthase subunit beta, mitochondrial (EC 3.6.3.14; UniProt P06576; also known as ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide, beta-F1-ATPase, Epididymis secretory protein Li 271, Mitochondrial ATP synthase beta subunit, Mitochondrial ATP synthetase beta subunit) is encoded by the ATP5B (also known as ATPMB, ATPSB, HEL-S-271) gene (Gene ID 506) in human. Mitochondrial ATP synthase produces ATP from ADP in the presence of a proton gradient generated by electron transport complexes. This ATPase contains two structural domains, F1-containing extramembrane catalytic core, and F0-containing the membrane proton channel that are linked via a central stalk and a peripheral stalk. Subunits alpha and beta form the catalytic code in F1. Tumor development requires the selection of cancer cells with a repressed biogenesis and functional activity of mitochondria. Both beta-F1-ATPase/GAPDH and beta-F1-ATPase/Hsp60 ratios are found to be significantly lower in tumors than the corresponding normal tissues. Studies conducted in human colon cancer cell line HCT116 show the involvement of AMPK (AMP-activated protein kinase) and GCN2 (general control non-derepressible 2; eIF2α kinase) in the onset of colon cancer progression by repressing of beta-F1-ATPase synthesis and promoting the abnormal bioenergetics of mitochondria.

Immunogen

His-tagged recombinant protein corresponding to human ATP Synthase subunit β.

Application

Immunocytochemistry Analysis: A representative lot immunostained mitochondrial tubular network in human breast cancer Hs578T cells (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
ELISA Analysis: A representative lot detected His-tagged full-length human ATP Synthase subunit β (beta-F1-ATPase) recombinant protein by direct ELISA (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
Dot Blot Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) by Dot blot using His-tagged full-length human beta-F1-ATPase recombinant protein or HepG2 lysate (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) in human hepatoma HepG2, murine hepatoma Hepa 1-6, and normal rat liver epithelial C9 (Clone 9) cells.
Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) expression in various cancer patients tissues (Acebo, P., et al (2009). Transl Oncol. 2(3):138-145).
Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) downregulation in HCT116 human colon cancer cells in response to AMPK pathway activation upon oligomycin or AICAR treatment (Martinez-Reyes, J., et al. (2012). Biochem J. 444(2):249-259).

Research Category
Signaling

Research Sub Category
Developmental Signaling

This Anti-ATP Synthase subunit β Antibody, clone 11/21-7-A8 is validated for use in Western Blotting, Immunocytochemistry, ELISA and Dot Blot for the detection of ATP Synthase subunit β.

Target description

~56 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATP Synthase subunit β in 10 µg of HepG2 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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