• USA Home
  • MABS148 - Anti-PKLR Antibody, clone 11F12.1

EMAIL THIS PAGE TO A FRIEND
MABS148 Sigma-Aldrich

Anti-PKLR Antibody, clone 11F12.1

Detect PKLR using this Anti-PKLR Antibody, clone 11F12.1 validated for use in Western Blotting & IHC.

Synonym: Pyruvate kinase isozymes R/L, Pyruvate kinase 1, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase

  •  eCl@ss 32160702

  •  NACRES NA.41

Purchase

Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 µL (MABS148)
application(s)   immunohistochemistry: suitable
  western blot: suitable
biological source   mouse
clone   11F12.1, monoclonal
  monoclonal
isotype   IgG2bκ
shipped in   wet ice
species reactivity   human, rat, mouse
gene symbol   PKLR(5313)
  PK1
  PKL

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

~60 kDa observed

Analysis Note

Control
HepG2 cell lysate

Application

Research Category
Metabolism

Research Sub Category
Insulin/Energy Signaling

Western Blot Analysis: A 1:1,000 dilution from a representative lot detecte PKLR in 10 µg of rat liver tissue lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected PKLR in rat liver hepatocytes.

General description

PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phosphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA).

Immunogen

GST-tagged recombinant protein corresponding to human PKLR.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected PKLR in 10 µg of HepG2 cell lysate.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?