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  • MABS148 - Anti-PKLR Antibody, clone 11F12.1

MABS148 Sigma-Aldrich

Anti-PKLR Antibody, clone 11F12.1

Detect PKLR using this Anti-PKLR Antibody, clone 11F12.1 validated for use in Western Blotting & IHC.

Synonym: Pyruvate kinase isozymes R/L, Pyruvate kinase 1, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase

  •  eCl@ss 32160702

  •  NACRES NA.41



Related Categories Antibodies, Primary Antibodies More...
packaging   100 µL (MABS148)
application(s)   immunohistochemistry: suitable
  western blot: suitable
biological source   mouse
clone   11F12.1, monoclonal
isotype   IgG2bκ
shipped in   wet ice
species reactivity   human, rat, mouse
gene symbol   PKLR(5313)



Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

~60 kDa observed

Analysis Note

HepG2 cell lysate


Research Category

Research Sub Category
Insulin/Energy Signaling

Western Blot Analysis: A 1:1,000 dilution from a representative lot detecte PKLR in 10 µg of rat liver tissue lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected PKLR in rat liver hepatocytes.

General description

PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phosphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA).


GST-tagged recombinant protein corresponding to human PKLR.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.


Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected PKLR in 10 µg of HepG2 cell lysate.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis

Protocols & Articles


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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