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  • MABS1691 - Anti-Goα Antibody, clone 3D9.1

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MABS1691 Sigma-Aldrich

Anti-Goα Antibody, clone 3D9.1

Detect Go (G(o)alpha) using this mouse monoclonal Anti-Go , clone 3D9.1, Cat. No. MABS1691, validated for use in Western Blotting.

Synonym: Guanine nucleotide-binding protein G(o) subunit alpha, G(o)alpha

  •  eCl@ss 32160702

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Properties

Related Categories Alphabetical Index, Antibodies, GM-GO, Primary Antibodies
clone   3D9.1, monoclonal
biological source   mouse
application(s)   western blot: suitable
species reactivity   mouse, rat, rat, mouse
species reactivity (predicted by homology)   human (based on 100% sequence homology)
shipped in   ambient
antibody form   culture supernatant
isotype   IgG1κ
antibody product type   primary antibodies
NCBI accession no.   NP_066268
UniProt accession no.   P29777
Gene Information   human ... GNAO1(2775)

Description

General description

Guanine nucleotide-binding protein G(o) subunit alpha (UniProt P29777; also known as G(o)alpha) is encoded by the GNAO1 (also known as EIEE17) gene (Gene ID 2775) in human. Heterotrimeric G proteins are activated by G protein-coupled receptors (GPCRs) and are composed of alpha ( ), beta ( ), and gamma ( ) subunits. There exists also monomeric Ras superfamily of small GTPases that are commonly referred to as small G-proteins. G-proteins mediate signal transduction initiated by GPCRs. Ligand binding activates GPCRs by inducing a conformational change. The activated GPCRs catalyzes the GDP-to-GTP exchange of the G subunit, causing a dissociation of the G dimer from the now GTP-bound G subunit. The G subunit gradually hydrolyzes the bound GTP to GDP and eventually re-associates with G and re-starts the cycle. There exist at least 20 different G subunits, which are separated into four main groups, Gi, Gs, Gq, and G12/13. Five types of Gi alpha subunits exist, Gi (GNAI1, GNAI2, and GNAI3), Go (GNAO1), Gt (GNAT1 and GNAT2), Ggust (GNAT3), and Gz (GNAZ). Gi/o family members inhibit adenylyl cyclase and can reduce neuronal excitability through regulation of ion channels. G(o)alpha plays a key role in presynaptic inhibition of neurotransmitter release through a G -mediated suppression of voltage-gated Ca2+ channels. G(o)alpha is the most abundant G o protein in the mammalian brain, defective G(o)alpha function can result in neurological impairments.

Specificity

Clone 3D9.1 was raised against a C-terminal region sequence derived from human Go (G(o)alpha) spliced isoform Alpha-1. Cross-reactivity toward human/mouse/rat spliced isoform Alpha-2 (~69% amino acid identity with spliced isoform Alpha-1) has not been confirmed.

Immunogen

KLH-conjugated linear peptide corresponding to a sequence from the C-terminal region of human Go (G(o)alpha).

Application

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Go (G(o)alpha) in 10 µg of mouse cerebellum, mouse hypothalamus, rat whole brain, and rat hippocampus tissue lysates.

Target description

~40 kDa observed. 39.92/39.96 kDa (human isoform Alpha-1/Alpha-2), 39.95/39.90 kDa (mouse isoform Alpha-1/Alpha-2), 39.94/39.95 kDa (rat isoform Alpha-1/Alpha-2) calculated (Met1 removed).

Physical form

Mouse monoclonal hybridoma culture supernatant without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.


Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:1000 dilution of this culture supernatant detected Go (G(o)alpha) in 10 µg of mouse whole brain tissue lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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Protocols

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