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MABS1894 Sigma-Aldrich

Anti-TAB-2 Antibody, clone 1D5.2

Anti-TAB-2, clone 1D5.2, Cat. No. MABS1894, is a mouse monoclonal antibody that detects TAK1-binding protein 2 and has been tested for use in Western Blotting.

Synonym: Mitogen-activated protein kinase kinase kinase 7-interacting protein 2, TAB-2, TAK1-binding protein 2, TGF-beta-activated kinase 1 and MAP3K7-binding protein 2, TGF-beta-activated kinase 1-binding protein 2

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Properties

Related Categories Antibodies, Primary Antibodies More...
format   Purified
molecular weight   ~77 kDa observed; 76.49 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
application(s)   Western Blotting
isotype   IgG2bκ
clone   Monoclonal Antibody
  1D5.2
purification method   Protein G purified
concentration   Please refer to lot specific datasheet.
host   Mouse
species reactivity   Human
NCBI accession no.   NP_001278963
UniProt accession no.   Q9NYJ8
gene symbol   TAB2(23118)
  KIAA0733
  MAP3K7IP2
material size   100 μg
shipped in   ambient
storage conditions   Stable for 1 year at 2-8°C from date of receipt.

Description

Background information

TGF-beta-activated kinase 1 and MAP3K7-binding protein 2 (UniProt: Q9NYJ8; also known as Mitogen-activated protein kinase kinase kinase 7-interacting protein 2, TAK1-binding protein 2, TAB-2, TGF-beta-activated kinase 1-binding protein 2) is encoded by the TAB2 (also known as KIAA0733, MAP3K7IP2) gene (Gene ID: 23118) in human. TAB-2 is a peripheral membrane protein that serves as an adapter protein that links MAP3K7/TAK1 and TRAF6. Two isoforms of TAB-2 have been reported that are produced by alternative splicing. TAB-2 promotes MAP3K7 activation in the IL-1 signaling pathway. Following IL-1 stimulation it translocates from the membrane to cytosol. TAB-2 is widely expressed. In the embryo, it is expressed in the ventricular trabeculae, endothelial cells of the conotruncal cushions of the outflow tract and in the endothelial cells lining the developing aortic valves. TAB-2 contains a RanBP2-type zinc finger domain (aa 663-693) that mediates binding to two consecutive Lysine 63-linked ubituitins. The binding of Lysine 63-linked polyubiquitin chains to TAB-2 promotes autophosphorylation of MAP3K7 at Threonine 187. Mutations in TAB2 gene are reported to cause multiple congenital heart defects characterized by congenital developmental abnormalities involving structures of the heart, including left ventricular outflow tract obstruction, sub-aortic stenosis, atrial fibrillation, bicuspid aortic valve and aortic dilation.

Physical form

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Application

Western Blotting Analysis: 1 µg/mL from a representative lot detected TAB-2 in 10 µg of Raji cell lysate.

Specificity

Clone 1D5.2 specifically detects human TGF-beta-activated kinase 1 and MAP3K7-binding protein 2. It targets an epitope within 133 amino acids from the C-terminal region.

Immunogen

GST/His-tagged recombinant fragment corresponding to 133 amino acids from the C-terminal region of human TAK1-binding protein 2 (TAB-2)

Species Reactivity Notes

Human.

Quality Assurance

Evaluated by Western Blotting in TF-1 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TAB-2 in 10 µg of TF-1 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles
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