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  • MABS192 - Anti-IGF-IR Antibody, clone αIR3 (Azide Free)

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MABS192 Sigma-Aldrich

Anti-IGF-IR Antibody, clone αIR3 (Azide Free)

clone αIR3, from mouse

Synonym: Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, IGF-I receptor, CD221

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, I-IG, Primary Antibodies
clone   αIR3, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
species reactivity   human
shipped in   dry ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_000866
UniProt accession no.   P08069
Gene Information   human ... IGF1R(3480)

Description

General description

The insulin-like growth factors (IGFs) or somatomedins are mitogenic peptides present in the plasma of human, rat, and other species. Two distinct but chemically related IGFs have been purified from human plasma: IGF-I (Somatomedin C) and IGF-II. They are single chain polypeptides with molecular weights of 7,640 and 7,471, respectively. Types I and II IGF receptors differ in their structure and in their peptide binding specificity. Type I IGF receptors are structurally homologous to insulin receptors and contain alpha-subunits (MW = 130,000) that bind peptide, and beta-subunits (MW = 90,000), which have intrinsic tyrosine kinase activity. The protein-tyrosine kinase activity of the insulin receptor and, presumably the IGF-I receptor, is required for insulin to stimulate all of its biological responses in cells. MABS192 is constituted as azide-free.

Immunogen

Partially purified human placenta IGF-1 receptor

Application

Anti-IGF-IR Antibody, clone αIR3 (Azide Free) is an antibody against IGF-IR for use in ICC.

Neutralization Assay Analysis: A representative lot detected IGF-IR in NEUT (Rohlik, Q. T., et al. (1987). Biochem Biophys Res Commun. 149(1):276-281; Kull, F. C. Jr., et al. (1983). 258(10):6561-6566.).

Research Category
Signaling

Research Sub Category
Cell Cycle, DNA Replication & Repair

Target description

155 kDa calculated

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected IGF-IR in HepG2 cells.

Analysis Note

Control
HepG2 cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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