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MABS275 Sigma-Aldrich

Anti-Gli3 Antibody, clone 5E1

clone 5E1, from mouse

Synonym: Transcriptional activator GLI3, GLI3 form of 190 kDa, GLI3-190, GLI3FL, GLI3 C-terminally truncated form, GLI3 form of 83 kDa, GLI3-83

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, GD-GL, Primary Antibodies
clone   5E1, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   human, mouse
shipped in   wet ice
isotype   IgMκ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_000159.3
UniProt accession no.   P10071
Gene Information   human ... GLI3(2737)

Description

General description

Gli3 can function as both a transcriptional activator and repressor of the sonic hedgehog (Shh) pathway. The full-length Gli3 form (GLI3FL) becomes an activator (GLI3A) after phosphorylation and nuclear translocation. The C-terminally truncated form (GLI3R), acts as a repressor. Proper balance between the Gli3 activator and the repressor specifies limb digit number and identity during development. In concert with TRPS1, Gli3 plays a role in activating chondrocyte proliferation. Gli3 is expressed in several normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Gli3 defects are associated with Greig cephalo-poly-syndactyly syndrome (GCPS), Pallister-Hall syndrome (PHS), polydactyly postaxial type A1 (PAPA1), polydactyly postaxial type B polydactyly (PAPB), and polydactyly preaxial type 4 (POP4).

Specificity

This antibody recognizes the truncated, repressor form and the full length protein of human Gli3.

Immunogen

Recombinant protein corresponding to the putative repressive motif of human GLI3.

Application

Immunocytochemistry Analysis: A representative lot detected Gli3 in PK-15, COS-1, and TM4 cells cotransfected with recombinant Gli3 (Hunt, R., et al. (2007). Hybridoma. 27(4):231-240.).

Immunohistochemistry Analysis: A representative lot detected Gli3 in mouse embryo cross-sections (Laht, S., et al. (2008). Hybridoma. 27(3):167-174.).

Research Category
Signaling

Research Sub Category
Developmental Signaling

This Gli3 antibody is validated for use in WB, ICC & IHC for the detection of the Gli3 protein.

Target description

~75 kDa and ~200 kDa observed. The truncated, repressor form and full length form of this protein has been observed at ~83 kDa and ~190 kDa, respectively (Tanimoto, Y., et al. (2012). J Biol Chem. 287(25):21429-21438.). Uncharacterized bands may be observed at ~160 kDa and below ~50 kDa in some cell lysates.

Physical form

Format: Purified

Purified by Ion-Exchange Chromatography

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in human thymus tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected in Gli3 in 10 µg of human thymus tissue lysate.

Analysis Note

Control
Human thymus tissue lysate

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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