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MABS278 Sigma-Aldrich

Anti-EMAPII Antibody, clone M7/1, (Preservat ive free)

Anti-EMAPII Antibody, clone M7/1, (Preservat ive free) detects level of EMAPII & has been published & validated for use in Western Blotting, Activity Assay & IHC.

Synonym: multisynthetase complex auxiliary component p43, multisynthetase complex auxiliary component 1, ARS-interacting multifunctional protein 1, Aminoacyl tRNA synthase complex-interacting multifunctional protein, Multisynthase complex auxiliary component p43,

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, EJ-EN, Primary Antibodies
clone   M7/1, monoclonal
biological source   rat
application(s)   activity assay: suitable
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   human, human, human
species reactivity (predicted by homology)   mouse (immunogen homology), mouse (immunogen homology)
shipped in   wet ice
isotype   IgG2aκ
antibody product type   primary antibodies
NCBI accession no.   NP_031952
UniProt accession no.   P31230

Description

General description

Endothelial monocyte-activating polypeptide II (EMAPII) is a 22 kDa pro-inflammatory cytokine that regulates TNFβ signaling. The mature EMAPII is formed from a functional EMAPII precursor protein in response to apoptosis, and other forms of cell stress. Previous studies have reported that it may decrease the growth of tumors by inhibiting angiogenesis. EMAPII has also been implicated in autoimmune diseases. However, EMAPII is also involved in a range of normal biological processes. It is a component of the multisynthase complex that binds tRNA and plays an essential role in protein translation, and may also play a role in lung development.

Specificity

This antibody recognizes the mature form of this protein at ~23 kDa and the larger pro EMAP II form at ~43 kDa which is secreted based on lysate.

Immunogen

His-tagged recombinant protein corresponding to mouse EMAPII.

Application

Research Category
Signaling

Research Sub Category
Developmental Signaling

Western Blot Analysis: A representative lot was used by an independent laboratory in recombinant EMAP II proteins. (Rajashekhar, G., et al. (2009). J Immunol Methods. 350(1-2):22–28.)

Neutralization Antibody: A representative lot was used by an independent laboratory to functionally neutralize pro and mature forms of EMAPII in lung tissue lysate. (Clauss, M., et al. (2012). J Clin Invest doi:10.1172/JCI43881.)

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in lung parenchyma of patients with emphysema. (Clauss, M., et al. (2012). J Clin Invest doi:10.1172/JCI43881.)

Target description

~43 kDa observed. This antibody recognizes the mature form of this protein at ~23 kDa and the larger pro EMAP II form at ~43 kDa which is secreted based on lysate.

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected EMAPII in 10 µg of HeLa cell lysate.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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