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MABS456 Sigma-Aldrich

Anti-PTP1B Antibody (Oxidized), clone scFvs 45

clone scFvs 45, from chicken

Synonym: Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B, PTP-1B

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, PT-PY, Primary Antibodies
clone   scFvs 45, monoclonal
biological source   chicken
application(s)   immunoprecipitation (IP): suitable
species reactivity   human
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_002818
UniProt accession no.   P18031
Gene Information   human ... PTPN1(5770)

Description

General description

PTP1B is the prototypic member of the protein tyrosine phosphatase (PTP) family, which comprises at least 37 proteins. The family is characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. The PTPs have high substrate specificity for phosphotyrosyl proteins. At the primary sequence level, PTPs share little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. PTP1B is a negative regulator of insulin and leptin signal transduction and may be a potential therapeutic target for diabetes and obesity. Insulin also influences the expression of splice variants of PTP1B.

Specificity

This antibody only reacts with the oxidized form.

Immunogen

Epitope: PTP1B-OX

Recombinant protein corresponding to the PTP1B-OX of human PTP1B (Oxidized).

Application

Anti-PTP1B Antibody (Oxidized), clone scFvs 45 | MABS456 is an antibody against PTP1B Antibody for use in Immunoprecipitation, Inhibits Activity/Function.

Immunoprecipitation Analysis: This antibody immunoprecipitated PTP1B (Oxidized) recombinant (aa 1-321; WT and CASA mutant) (Haque, A., et al. (2011). Cell. 147:185-198).
Immunoprecipitation Analysis: This antibody immunoprecipitated PTP1B (Oxidized) recombinant (aa 1-321; WT and CASA mutant) (Haque, A., et al. (2012). Nature America. 7(12):2127-2143).
Inhibits Activity/Function Analysis: A representative lot detected PTP1B (Oxidized) in recombinant PTP1B (aa 1-321; WT and CASA mutant) and scFv45) (Haque, A., et al. (2011). Cell. 147:185-198).
Inhibits Activity/Function Analysis: A representative lot detected PTP1B (Oxidized) in recombinant PTP1B (aa 1-321; WT and CASA mutant)and scFv45) (Haque, A., et al. (2012). Nature America. 7(12):2127-2143).

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Target description

~37 kDa observed. The calculated molecular weight is 50kDa however PTP1B has been observed at ~37 kDa since it is a truncated form (aa 1-321). Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Ni-NTA agarose

Purified chicken monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Immunoprecipitation in recombinant PTP1B (aa 1-321; WT and CASA mutant) and scFv45 cell lysate.

Immunoprecipitation Analysis: 278 ng of this antibody detected PTP1B (Oxidized) in recombinant PTP1B (aa 1-321; WT and CASA mutant) and scFv45 cell lysate.

Linkage

Replaces: 04-1140

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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