• USA Home
  • MABS784 - Anti-Fatty acid synthase, clone EPR7465, Rabbit Monoclonal Antibody

EMAIL THIS PAGE TO A FRIEND
MABS784 Sigma-Aldrich

Anti-Fatty acid synthase, clone EPR7465, Rabbit Monoclonal Antibody

This Anti-Fatty acid synthase antibody is validated for use in WB, IC for the detection of Fatty acid synthase.

Synonym: Fatty acid synthase, FASN, FAS

  •  eCl@ss 32160702

  •  NACRES NA.41

Purchase

Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 µL (MABS784)
application(s)   immunocytochemistry: suitable
  western blot: suitable
biological source   rabbit
clone   EPR7465, monoclonal
isotype   IgG
shipped in   wet ice
species reactivity   human
gene symbol   FAS(355)
  FASN(2194)
format   Unpurified
molecular weight   ~273 kDa observed. Uncharacterized bands may be observed in some lysate(s).
clone   Monoclonal Antibody
concentration   Please refer to lot specific datasheet.
Quality Assurance   Evaluated by Western Blotting in HeLa, HEK293, A549, SHSY-5Y, and MOLT4 cell lysates.

Western Blotting Analysis: A 1:1,000-10,000 dilution of this antibody detected Fatty acid synthase in HeLa, HEK293, A549, SHSY-5Y, and MOLT4 cell lysates.

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

~273 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Application

Immunocytochemistry Analysis: A 1:250-500 dilution from a representative lot detected Fatty acid synthase in A549 cells.

Produced in collaboration with Epitomics

Research Category
Signaling

Research Sub Category
Lipid Metabolism & Weight Regulation

General description

Fatty acid synthase (FASN or FAS) is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, Fatty acid synthase has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. Fatty acid synthase is localized in the cytoplasm and is expressed in brain, lung, and liver.

Immunogen

Synthetic peptide corresponding to human Fatty acid synthase.

Other Notes

Concentration: Please refer to lot specific datasheet.

Specificity

Equilibrium disassociation constant (KD) is 1.58 x 10 -11 M

Physical form

Format: Unpurified

Rabbit monoclonal in buffer containing PBS with up to 0.1% sodium azide, 0.05% BSA, and 50% glycerol.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?