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MABT117 Sigma-Aldrich

Anti-Endoglin (CD105) Antibody, clone 44G4

Anti-Endoglin (CD105) Antibody, clone 44G4 is an antibody against Endoglin (CD105) for use in FC, IH & IP.

Synonym: Endoglin, CD104

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, EJ-EN, Primary Antibodies
clone   44G4, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
species reactivity   human
shipped in   wet ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_000109
UniProt accession no.   P17813

Description

General description

Endoglin (CD105) is a homodimeric integral membrane glycoprotein comprised of a 95 kDa subunit. Endoglin is a component of the TGF-β receptor system that functions as an ancillary receptor influencing binding of the Transforming Growth Factor beta (TGF-β) family of ligands to signaling receptors. Endoglin binds to TGF-β1 and TGF-β3 with high affinity, but does not bind TGF-β2. Endoglin is expressed by vascular endothelial cells and syncytiotrophoblasts of placenta and is weakly expressed by stromalfibroblasts. Endoglin is also expressed by U937 cells and by activated macrophages. Endoglin expression is up-regulated on activated endothelium in tissues undergoing angiogenesis, such as in tumors, or in cases of wound healing or dermal inflammation.

Immunogen

Whole HOON Pre-B Leukemic Cells

Application

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory to detect Endoglin in normal human lymph node, tonsil, spleen thrymus, liver, breast, lung, and kidney specimens (Gougos, A., and Letarte, M. (1988). J Immunol. 141(6):1925-1933).

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to IP Endoglin from HOON, NALM-6, 3104, 3161, HL-60, U-937, EM-3 and KG-1a cells (Gougos, A., and Letarte, M. (1988). J Immunol. 141(6):1925-1933).

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Target description

71 kDa calculated

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Flow Cytometry in HUVEC cells.

Flow Cytometry Analysis: A 1:200 dilution of this antibody detected Endoglin in HUVEC cells.

Analysis Note

Control
HUVEC cells

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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