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  • MABT123 - Anti-Pirh2 Antibody, clone 11G8.1

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MABT123 Sigma-Aldrich

Anti-Pirh2 Antibody, clone 11G8.1

clone 11G8.1, from mouse

Synonym: RING finger and CHY zinc finger domain-containing protein 1, Androgen receptor N-terminal-interacting protein, CH-rich-interacting match with PLAG1, E3 ubiquitin-protein ligase Pirh2, RING finger protein 199, Zinc finger protein 363, p53-induced RING-H2

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, PH-PI, Primary Antibodies
clone   11G8.1, monoclonal
biological source   mouse
application(s)   western blot: suitable
species reactivity   mouse, human
shipped in   wet ice
isotype   IgG2aκ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_056251
UniProt accession no.   Q96PM5
Gene Information   human ... RCHY1(25898)

Description

General description

Pirh2, also known as Androgen receptor N-terminal-interacting protein or CH-rich-interacting match with PLAG1 or E3 ubiquitin-protein ligase Pirh2 or RING finger protein 199 or Zinc finger protein 363 or p53-induced RING-H2 protein (Pirh2), encoded by the gene RCHY1/ARNIP/CHIMP/PIRH2/RNF199/ZNF363, is an E3 ubiquitin ligase protein that mediates the E3-dependent ubiquitination and proteasomal degradation of a number of important signaling and DNA methylation proteins including p53/TP53, p73, HDAC1 and CDKN1B. Pirh2 also monoubiquitinates the translesion DNA polymerase POLH and contributes to the regulation of the cell cycle progression. Additionally, Pirh2 increases androgen receptor transcription factor activity as well and Interacts with AR, p53/TP53, MDM2, HDAC1, KAT5, PLAG1, PLAGL2, CDKN1B, COPE, UBE2D2 and GORAB/NTKLBP1 proteins. Pirh2 is localized to the cytoplasm and nucleus, and can be found in nucleus speckles. Pirh2 is expressed in all cell types examined and is up-regulated during the S phase of the cell cycle. Pirh2 is expressed at low levels during G phase.

Immunogen

GST-tagged recombinant protein corresponding to human Pirh2.

Application

Anti-Pirh2 Antibody, clone 11G8.1 is a highly specific mouse monoclonal antibody & that targets Pirh2 & has been tested in western blotting.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Target description

~30 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in H1299 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Pirh2 in 10 µg of H1299 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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