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MABT20 Sigma-Aldrich

Anti-OL-protocadherin Antibody, clone 5G10

Anti-OL-protocadherin Antibody, clone 5G10 is an antibody against OL-protocadherin for use in WB, IC, IH.

Synonym: protocadherin 10, ortholog of OL-pcdh

  •  eCl@ss 32160702

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Properties

Related Categories Antibodies, Primary Antibodies More...
gene symbol   OL-PCDH
  Pcadh10
  Pcdh10
NCBI accession no.   NP_001091641
UniProt accession no.   Q925I8
Quality Assurance   Evaluated by Western Blot in mouse E13-E14 brain tissue lysate.

Western Blot Analysis: 1 µg/mL of the antibody detected OL-protocadherin in 10 µg of mouse E13-E14 brain tissue lysate.
application(s)   Western Blotting, Immunocytochemistry, Immunohistochemistry
clone   5G10
  Monoclonal Antibody
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
format   Purified
host   Rat
isotype   IgG2aκ
molecular weight   ~ 130 kDa observed
packaging   100 µg (MABT200)
shipped in   wet ice
species reactivity   Mouse

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
Mouse E13-E14 brain tissue lysate

Application

Immunofluorescence Analysis: The antibody has been shown to detect OL-protocadherin in IF. (Nakao, 2008).

Immunohistochemistry Analysis: The antibody has been shown to detect OL-protocadherin in IH. (Aoki, 2003).

Research Category
Cell Structure

Neuroscience

Research Sub Category
Adhesion (CAMs)

Synapse & Synaptic Biology

General description

OL-protocadherin (OL-pc, protocadherin 10) is a transmembrane protein and a member of the cadherin superfamily, which is characterized by its members’ abilities to undergo interactions that are homophilic in nature via their extracellular domain. Several protocadherins have been observed within the nervous system, and a few have been shown to localize at synapses. With OL-pc, the homophilic binding has been shown to stimulate cell aggregation when the protein is introduced into cadherin-deficient cells. Although the function of OL-protocadherin has yet to be elucidated, studies have shown OL-pc to be necessary to axonal normal projection through the ventral telencephalon, where OL-pc shows highest expression at the striatal region.

Immunogen

Epitope: Cytoplasmic domain

GST-tagged recombinant protein corresponding to the cytoplasmic domain of mouse OL-protocadherin.

Specificity

The antibody recognizes the cytoplasmic domain of OL-protocadherin.

Physical form

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Making Headway
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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