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  • MABT213 - Anti-SHP-1 Antibody, clone HG213, rabbit monoclonal

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MABT213 Sigma-Aldrich

Anti-SHP-1 Antibody, clone HG213, rabbit monoclonal

Anti-SHP-1 Antibody, clone HG213 detects level of SHP-1 & has been published & validated for use in WB.

Synonym: Tyrosine-protein phosphatase non-receptor type 6, Hematopoietic cell protein-tyrosine phosphatase, Protein-tyrosine phosphatase 1C, PTP-1C, Protein-tyrosine phosphatase SHP-1, SH-PTP1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SG-SK
conjugate   unconjugated
clone   HG213, monoclonal
biological source   rabbit
application(s)   western blot: suitable
species reactivity   human
shipped in   wet ice
antibody product type   primary antibodies
NCBI accession no.   NP_002822
UniProt accession no.   P29350

Description

General description

Src Homology region 2 domain Phosphatase 1 (SHP-1) is also known as Protein Tyrosine Phosphatase 1C (PTP1C), Protein Tyrosine Phosphatase non-receptor type 6 (PTPN6), and Hematopoietic Cell Phosphatase (HCPH). SHP-1 is a SH2 domain-containing cytosolic tyrosine phosphatase characterized by a catalytic phosphatase domain of approximately 240 amino acids that selectively dephosphorylates tyrosine residues in proteins; the SH2 domains are critical for SHP-1 to bind to ITIM domains in inhibitory immunoreceptors. SHP-1 is highly expressed in hematopoietic tissues and has a negative impact on lymphocyte signaling. SHP-1 is implicated in the control of tyrosine kinase signaling pathways in cellular proliferation, with a potential role in cancer.

Immunogen

Full-length glutathione transferase SHP-1 fusion protein, purified from bacteria.

Application

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

Target description

~67 kDa observed.
3 isoforms exist with molecular weights between 63 kDa and 68 kDa

Physical form

Rabbit monoclonal supernatant without sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected SHP-1 in 10 µg of Jurkat cell lysate.

Analysis Note

Control
Jurkat cell lysate

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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