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  • MABT386 - Anti-Hu GBP-1/GBP-1 Antibody, clone 6C5.1

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MABT386 Sigma-Aldrich

Anti-Hu GBP-1/GBP-1 Antibody, clone 6C5.1

clone 6C5.1, from mouse

Synonym: Interferon-induced guanylate-binding protein 1, GTP-binding protein 1, GBP-1, HuGBP-1, Guanine nucleotide-binding protein 1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, HU-HZ, Primary Antibodies
clone   6C5.1, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable (paraffin)
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG2aκ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_002044
UniProt accession no.   P32455
Gene Information   human ... GBP1(2633)

Description

General description

mgbp-2/hGBP-1, or also known as Interferon-induced guanylate-binding protein 1, GTP-binding protein 1 (GBP-1/HuGBP-1), or Guanine nucleotide-binding protein 1, and encoded by the gene GBP1, is another enzyme important in innate defense. Among its functions, this enzyme hydrolyzes GTP to GMP and appears to promote oxidative killing and deliver antimicrobial peptides to autophagolysosomes, thus providing broad host protection against different pathogen classes. mgbp-2/hGBP-1 is localized to the cytoplasm but is also secreted in endothelial cells and localizes to the cytoplasmic side of the Golgi apparatus in expressing most cells. Interestingly, mgbp-2/hGBP-1is secreted by the endothelial cells in the CNS upon bacterial challenge even when not induced by gamma interferon. The enzyme is also induced by TNF-alpha and IL-1beta expression by immune cells and is considered a proinflammatory response factor.

Immunogen

GST-tagged recombinant protein corresponding to hu GBP-1/GBP-1.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected hu GBP-1/GBP-1 in human spleen tissues and rat brain tissue.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

This Anti-Hu GBP-1/GBP-1 Antibody, clone 6C5.1 is validated for use in western blotting & IHC (Paraffin) for the detection of Hu GBP-1/GBP-1.

Target description

~68 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in human spleen tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected hu GBP-1/GBP-1 in 400 µg of human spleen tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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