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  • MABT506 - Anti-ARP2, clone EPR7980, Rabbit Monoclonal Antibody

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MABT506 Sigma-Aldrich

Anti-ARP2, clone EPR7980, Rabbit Monoclonal Antibody

This Anti-ARP2 antibody is validated for use in WB, IH for the detection of ARP2.

Synonym: Actin-related protein 2, Actin-like protein 2, ACTR2, ARP2

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories AR-AR, Alphabetical Index, Antibodies, Primary Antibodies
clone   EPR7980, monoclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
UniProt accession no.   P61160
Gene Information   human ... ACTR2(10097)

Description

General description

Actin-related protein 2, also known as Actin-like protein 2, and encoded by the gene name ACTR2 or ARP2, functions as an ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. ARP2 is localized to the cytoplasm.

Specificity

Dissociation constant (KD) is 7.00 x 10-11 M

Application

Immunohistochemistry Analysis: A 1:100-250 dilution from a representative lot detected ARP2 in human uterus.

Produced in collaboration with Epitomics

Target description

~37 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Unpurified

Quality

Evaluated by Western Blotting in Jurkat, HUVEC, and MCF7 cell lysates.

Western Blotting Analysis: A 1:1,000-10,000 dilution of this antibody detected ARP2 in Jurkat, HUVEC, and MCF7 cell lysates.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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