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  • MABT508 - Anti-ARP2, clone EPR7979, Rabbit Monoclonal Antibody

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MABT508 Sigma-Aldrich

Anti-ARP2, clone EPR7979, Rabbit Monoclonal Antibody

This Anti-ARP2 antibody is validated for use in WB, FC for the detection of ARP2.

Synonym: Actin-related protein 2, Actin-like protein 2, ACTR2, ARP2

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories AR-AR, Alphabetical Index, Antibodies, Primary Antibodies
clone   EPR7979, monoclonal
biological source   rabbit
application(s)   flow cytometry: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG
antibody product type   primary antibodies
UniProt accession no.   P61160
Gene Information   human ... ACTR2(10097)

Description

General description

Actin-related protein 2, also known as Actin-like protein 2, and encoded by the gene name ACTR2 or ARP2, functions as an ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. ARP2 is localized to the cytoplasm.

Specificity

Dissociation constant (KD) is 1.75 x 10-11 M

Immunogen

Synthetic peptide corresponding to human ARP2.

Application

Flow Cytometry Analysis: A 1:10-100 dilution from a representative lot detected ARP2 in MCF-7 cells.

Produced in collaboration with Epitomics

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Target description

~37 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Unpurified

Rabbit monoclonal in buffer containing PBS with up to 0.1% sodium azide, 0.05% BSA, and 50% glycerol.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in Jurkat, HUVEC, MCF-7, and HeLa cell lysates.

Western Blotting Analysis: A 1:1,000-10,000 dilution of this antibody detected ARP2 in Jurkat, HUVEC, MCF-7, and HeLa cell lysates.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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