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  • MAC132 - Mouse Anti-Human IgA, Secretory Antibody, Clone 4G10.1

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MAC132 Sigma-Aldrich

Mouse Anti-Human IgA, Secretory Antibody, Clone 4G10.1

clone 4G10.1, from mouse

  •  eCl@ss 32160702

  •  NACRES NA.46

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Properties

Related Categories Antibodies, Human IgA Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies,
Quality Level   100
biological source   mouse
antibody product type   secondary antibodies
clone   4G10.1, monoclonal
species reactivity   human, human (IgA)
application(s)   ELISA: suitable
isotype   IgG1κ
shipped in   wet ice

Description

General description

The Mouse Anti-Human IgA antibody is an IgG monomeric immunoglobulin, built of two heavy chains and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). It is affinity-purified and demonstrated to react with Human IgA with minimal or no cross-reaction to Human IgG or Human IgM. The target of this antibody, Human IgA, constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B-cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA.

Specificity

Based on ELISA analysis, this antibody recognizes human IgA secretory subunit, with minimal or no cross-reaction human IgG or IgM.

Immunogen

Purified antibody corresponding to Human IgA, Secretory.

Application

ELISA. Can be used as a capture antibody for ELISA at 10 μg/ml in PBS without carrier protein. Antibody should be titrated for optimal results in individual systems.

Research Category
Secondary & Control Antibodies

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

This Mouse Anti-Human IgA, Secretory Antibody, Clone 4G10.1 is validated for use in Enzyme Immunoassay (ELISA) for the detection of Mouse Human IgA, Secretory.

Quality

Evaluated by ELISA against Human IgA, Human IgG and Human IgM

Linkage

Replaces: 411423-400UG

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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