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  • MAC142 - Anti-Cas9 Antibody, N-terminal Antibody, clone 6H4 Antibody, S. aureus

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MAC142 Sigma-Aldrich

Anti-Cas9 Antibody, N-terminal Antibody, clone 6H4 Antibody, S. aureus

clone 6H4, from mouse

Synonym: SaCas9, CRISPR-associated endonuclease Cas-9

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, CAM-CB, Primary Antibodies
clone   6H4, monoclonal
biological source   mouse
application(s)   immunofluorescence: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   bacteria, Staphylococcus aureus
species reactivity (predicted by homology)   all
shipped in   ambient
packaging   antibody small pack of 25 μg
isotype   IgG2bκ
Quality Level   100
antibody product type   primary antibodies
UniProt accession no.   J7RUA5

Description

General description

CRISPR-associated endonuclease Cas9 (UniProt: J7RUA5; also known as SaCas9) is encoded by the Cas9 gene (Gene ID: in Staphylococcus aureus. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer.Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self-versus non-self, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity. Cas9 endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed 3′-5′ exonucleolytically.

Specificity

Clone 6H4 detects Cas-9 in Staphylococcus aureus.

Immunogen

Epitope: N-terminus

His-tagged recombiant Cas-9 from Staphylococcus aureus.

Application

Anti-Cas-9, N-terminal, clone 6H4, Cat. No. MAC142, is a mouse monoclonal antibody that detects Cas-9 in Staphylococcus aureus and its identity has been confimed by Isotyping Test and Western Blotting.

Research Category
Secondary & Control Antibodies

Western Blotting Analysis: 0.57 µg/mL from a representative lot detected CRISPR-associated endonuclease Cas9/Csn1 in whole cell lysates of HEK293 (WT), HEK293/Cas9 from S. aureus, or HeLa/Cas9 from S. pyogenes. (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected CRISPR-associated endonuclease Cas9/Csn1 in transiently transfected U20S cells expressing SaCas9-T2A-GFP. (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

Immunoprecipitation Analysis: 1-2 µg from a representative lot detected CRISPR-associated endonuclease Cas9/Csn1 in SaCas9. (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

Target description

~124 kDa observed; 123.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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