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MCSP24H48 Millipore

Millicell® Hanging Cell Culture Insert, PET 3 μm, 24-well, 48/pk

Polyethylene Terephthalate hanging cell culture insert with pore size of 3 μm used in a 24-well plate for cell attachment, cell culture, cell differentiation & ICC.

Synonym: cell culture inserts, permeable culture insert, tissue culture insert, tissue culture plate

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Properties

Related Categories Cell Culture Supplies, Culture Plate Inserts, Labware, Millicell Hanging Cell Culture Inserts, Millicell inserts,
Quality Level   200
material   PET membrane
  polystyrene housing
sterility   sterile
packaging   pack of 48 ea (individually blister packaged)
parameter   50 °C max. temp.
filter diam.   6.5 mm
filtration area   0.3 cm2
size   24 wells
pore size   3.0 μm
input   sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type neural stem cell(s)
sample type cardiac stem cell(s)
surface area   0.3 cm2

Description

General description

Polyethylene Terephthalate (PET) hanging cell culture insert with pore size of 3.0 μm used in a 24-well plate for cell attachment, cell culture, cell differentiation & ICC.Device configuration: 24-well plate, 3μm pore sizeMillicell® cell culture inserts are an easy to use and convenient device for adherent or suspension cells. Allowing the access of media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.
Millicell® Hanging Inserts: Preloaded in receivers or available standalone
• For co-culturing and permeability assays
• Unique design allows easier basolateral access than other hanging inserts with less risk of contamination
• Available in 5 pore sizes and 3 diameters, including a 1 μm pore size that is optically transparent for better visualization by microscopy
• Available preloaded in 24-well receiver plates or as individual inserts in 3 diameters

Applications: Cell Attachment, Cell Growth, Cell Differentiation, Immunocytochemistry

Application

Polyethylene Terephthalate hanging cell culture insert with pore size of 3 μm used in a 24-well plate for cell attachment, cell culture, cell differentiation & ICC.

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

General Immunofluorescent Protocol for Millicell® Products

Immunocytochemical staining is a technique employing fluorescently labeled antibody, by which cells can be localized, labeled, and examined via fluorescent microscopy.
Keywords: Cell culture, Culture media, Fluorescent microscopy, Microscopy

Microscopic Examination of Samples

Millicell® devices using PET or CM membrane have been designed to allow visualization of cells from below using an inverted microscope. For viewing live cells, microscopic observations can be made th...
Keywords: Cell culture, Dehydration reaction, Immunofluorescence, Polymerization reactions, Positron Emission Tomography, Substitutions

Toluidine Blue Staining Protocol for Millicell

From our library of Articles, Sigma-Aldrich presents Toluidine Blue Staining Protocol for Millicell

Protocols

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

Note: Depending on the cell lines and the nature of the co-culture, the researcher can decide which side of the membrane is seeded first. To maintain sterile incubations of cells seeded on the unders...
Keywords: Cell culture

ECM Coating Protocols for Millicell

Coating of membranes and plastic surfaces with extra cellular matrices (ECMs) promotes cell attachment and monolayer formation. We have developed protocols for four types of ECMs on Millicell®-CM ins...
Keywords: Cell attachment, Cell culture, Condensations

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

Note: This protocol is designed to grow undifferentiated embryonic stem cells in an indirect co culture with the fibroblast feeder layer. Although it is targeted for use with Millicell®-24 and Millic...
Keywords: Cell culture, Positron Emission Tomography

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