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MECY2MAG-73K Millipore

MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel II, MECY2MAG-73K

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

  •  eCl@ss 32161000


To order a Milliplex kit, please search for your analyte of interest.




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Properties

Quality Level   200
species reactivity   mouse
mfr. no.   MILLIPLEX®
assay range   accuracy: 77-105%
  standard curve range: 1-5,000 pg/mL
(IFNγ)
  standard curve range: 12-50,000 pg/mL
(Exodus-2/CCL21/6Ckine)
  standard curve range: 12-50,000 pg/mL
(IFNβ1)
  standard curve range: 12-50,000 pg/mL
(IL-11)
  standard curve range: 12-50,000 pg/mL
(IL-20)
  standard curve range: 12-50,000 pg/mL
(MIP-3β/CCL19)
  standard curve range: 2-10,000 pg/mL
(IL-16)
  standard curve range: 2-10,000 pg/mL
(MCP-5/CCL12)
  standard curve range: 2-10,000 pg/mL
(MDC/CCL22)
  standard curve range: 2-10,000 pg/mL
(MIP-3α/CCL20)
  standard curve range: 2-10,000 pg/mL
(TARC/CCL17)
  standard curve range: 2-10,000 pg/mL
(TIMP-1)
  standard curve range: 24-100,000 pg/mL
(Fractalkine/CX3CL1)
  standard curve range: 24-100,000 pg/mL
(IL-17A/F)
  standard curve range: 5-20,000 pg/mL
(Erythropoietin (EPO))
application(s)   multiplexing: suitable
detection method   fluorescent (Luminex xMAP)
shipped in   ambient

Description

General description

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process. In addition the mouse model and mouse-to-human translational studies provide data essential to understanding the role of cytokines and immune response in both normal and disease states.

Panel Type: MAGNETIC Cytokines/Chemokines

The MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel II, contains all the components necessary to measure the following 15 biomarkers in any combination using Luminex xMAP technology: Erythropoietin (EPO), Exodus-2/CCL21/6Ckine, Fractalkine/CX3CL1, IFNβ1, IFNγ, IL-11, IL-16, IL-17A/F, IL-20, MCP-5/CCL12, MDC/CCL22, MIP-3α/CCL20, MIP-3β/CCL19, TARC /CCL17, TIMP-1. The kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 serum, plasma, or cell/tissue culture samples in duplicate.

Specificity

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Application

Research Category
Inflammation & Immunology

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

This assay is an overnight assay (16 hour incubation) or same day assay (2 hour incubation). Serum and plasma samples require (recommend) a 1:2 dilution with Assay Buffer. Each well requires 25 μL of sample, standard or control. Tissue culture supernatants can also be used and if needed, make appropriate dilutions with control medium.

Used to detect/quantify: Erythropoietin (EPO)

Exodus-2

Fractalkine/CX3CL1

IFNβ1

IFNγ

IL-11

IL-16

IL-17A/F

IL-20

MCP-5/CCL12

MDC (CCL22)

MIP-3α/CCL20

MIP-3β/CCL19

TARC/CCL17

TIMP-1

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

Everything you need in a single kit.

Components

Mouse Cytokine/Chemokine Panel II Standard, 1 vial, lyophilized

Mouse Cytokine/Chemokine Panel II Quality Control 1, 1 vial, lyophilized

Mouse Cytokine/Chemokine Panel II Quality Control 2, 1 vial, lyophilized

Serum Matrix (0.08% Sodium Azide), 1 vial, lyophylized

Assay Buffer, 1 bottle, 30 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Mouse Cytokine/Chemokine Panel II Detection Antibodies, 1 bottle, 3.2 mL

Mouse Cytokine/Chemokine Panel II Antibody-Immobilized Beads (50X), 90 µL ea

Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate

2 Foil Plate Sealers

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please contact Technical Service for linearity of dilution.

Sensitivity: Refer to kit protocol for sensitivities of individual cytokines/chemokines.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?