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MGAMMAG-300K Millipore

MILLIPLEX MAP Mouse Immunoglobulin Isotyping Magnetic Bead Panel - Isotyping Multiplex Assay

Isotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulins (Ig) in murine serum, plasma and cell culture samples.

  •  eCl@ss 32161000


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Properties

Quality Level   200
species reactivity   mouse
mfr. no.   MILLIPLEX®
assay range   accuracy: 100%
(IgM)
  accuracy: 85%
(IgG3)
  accuracy: 98%
(IgA)
  accuracy: 98%
(IgG2b)
  sensitivity: 0.18 ng/mL
(IgG2b)
  sensitivity: 0.32 ng/mL
(IgG3)
  sensitivity: 0.67 ng/mL
(IgG2a)
  sensitivity: 0.7 ng/mL
(IgG1)
  sensitivity: 1.1 ng/mL
(IgA)
  sensitivity: 1.73 ng/mL
(IgM)
  standard curve range: 1-900 ng/mL
(IgG2b)
  standard curve range: 2-1,500 ng/mL
(IgG1)
  standard curve range: 2-1,500 ng/mL
(IgG3)
  standard curve range: 2-1,500 ng/mL
(IgM)
  standard curve range: 3-2,000 ng/mL
(IgG2a)
  standard curve range: 5-3,000 ng/mL
(IgA)
application(s)   multiplexing: suitable
assay range   inter-assay cv: 14-17%
intra-assay cv: 5-10%
detection method   fluorescent (Luminex xMAP)
shipped in   wet ice

Description

General description

MILLIPLEX MAP Mouse Isotyping Magnetic Bead Panel, measuring mouse IgG subclasses (1, 2a, 2b, 3), IgM, and IgA, has been designed to enable you to measure both classes and subclasses accurately – all in one well. In addition this panel allows you to choose only those immunoglobulins you need for your experiments.

Produced by plasma cells and lymphocytes, immunoglobulins (antibodies) are critically involved in immune response, attaching to antigens and playing a role in their destruction. Immunoglobulins (Ig) can be classified by isotype, classes that differ in function and antigen response due to structure variability. Six major isotypes have been identified in placental mammals: IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA – all found in normal individuals. Immunoglobulin-deficiency disorders, such as autoimmune disease, some GI conditions and malignancies, are characterized by specific isotype deficiencies or varying concentrations of one or more isotypes. Disease states can range from the absence of one isotype class or subclass to a total deficiency of immunoglobulin classes.

Application

• This kit may be used for the analysis of IgG1, IgG2a, IgG2b, IgG3, IgA and IgM in mouse serum or tissue/cell lysate and culture supernatant samples.
• There are 2 one-hour room-temperature incubations.
• This assay requires 50 µl of 1:50,000 diluted plasma or serum, or 50 μL cell culture supernatant diluted to approximately 1μg/mL Ig per well.

Used to detect/quantify: IgG1

IgG2a

IgG2b

IgG3

IgA

IgM

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Linkage

Replaces: MGAM-300

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
Peer-Reviewed Papers
15

References

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