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MXSMEND48 Millipore

Milliflex Cassette prefilled with m-Endo LES Agar

This medium is designed for the recovery of coliform organisms

  •  eCl@ss 32160801

  •  NACRES NA.74

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Properties

Related Categories Microbiological Culture Media, Microbiology, Ready Media - Plates More...
Quality Level   300
form   plate (pre-poured agar)
shelf life   6 mo.
feature   ready-to-use
packaging   cassette of 10 mL
  pack of 48 tests
mfr. no.   Milliflex®
application(s)   bioburden testing: suitable
  microbiology: suitable
  water monitoring: suitable
color   slightly opalescent rose
input   sample type pharmaceutical(s)
sample type cosmetics
sample type bottled water
sample type beer
pH   7.2±0.2 (25.0 °C)
Featured Industry   Cosmetics
Pharmaceutical
compatibility   for use with Milliflex®
shipped in   ambient
storage temp.   2-8°C
suitability   coliforms
storage conditions   Right side up, protected from light.
  2 to 8 °C

Description

General description

Milliflex® Cassette are ready to use agar-based media to be used with Milliflex® or Milliflex Oasis® system for convenient, rapid and reliable bioburden testing. The prefilled agar cassette helps to streamline the microbial contamination testing workflow with quality assurance and avoiding risks of exogenous contamination. Each plate is a 3-piece assembly containing approximately 10ml of the media. m-Endo LES Agar is a selective media for detecting total coliforms in drinking water. Sodium lauryl sulphate and sodium desoxycholate are inhibitors of gram-positive bacteria, basic Fuchsin is a pH indicator, sodium sulphite is added to decolorize the basic fuchsin solution and the ethanol additive increases the antibacterial nature of the formulation. Lactose fermenting bacteria produce acetaldehyde that reacts with the sodium sulphite and fuchsin to form red colonies. The development of a metallic sheen occurs when the organism produces aldehydes with the rapid fermentation of lactose. The sheen will be suppressed if the inoculum is too heavy.

Application

Milliflex® Cassette prefilled with m-Endo LES Agar is used in Bioburden testing and general microbiological analysis of water, wastewater and dairy products. It is widely used in pharmaceutical, cosmetic and brewing industry.

Packaging

48 ea in 10 mL Agar in plastic Cassette

Culture Medium

m-Endo LES

Analysis Note

Coliform colonies appear deep red with distinct green metallic sheen

QC organisms
E. coli (ATCC 25922)

P. aeruginosa (ATCC 9027)

E. aerogenes (ATCC 49701)

S. aureus (ATCC 6538) as negative control

Target Microorganisms: Coliform species

Legal Information

MILLIFLEX OASIS is a registered trademark of Merck KGaA, Darmstadt, Germany

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Milliflex® and Milliflex Oasis® Solid Culture Media and Cassettes

We offer a full range of ready-to-use agar media cassettes manufactured according to ISO certified processes with high purity ingredients for long life to use with the Milliflex Oasis® system for bio...
Keywords: Aerobic, Anaerobic

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