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MXSMHPC48 Millipore

Milliflex Cassette prefilled with Heterotrophic Plate Count Agar

This medium is used for the recovery of stressed heterotrophic plate count bacteria found in various types of waters, especially high purity water

  •  eCl@ss 32160801

  •  NACRES NA.74



Related Categories Microbiological Culture Media, Microbiology, Ready Media - Plates More...
Quality Level   300
form   plate (pre-poured agar)
shelf life   6 mo.
feature   ready-to-use
packaging   cassette of 10 mL
  pack of 48 tests
mfr. no.   Milliflex®
application(s)   bioburden testing: suitable
  microbiology: suitable
  water monitoring: suitable
color   clear tan to light amber
input   sample type beer
sample type cosmetics
sample type bottled water
sample type pharmaceutical(s)
pH   7.1±0.2 (25.0 °C)
Featured Industry   Cosmetics
compatibility   for use with Milliflex®
shipped in   ambient
storage temp.   2-8°C
suitability   heterotrophic bacteria
  heterotrophic bacteria
storage conditions   Right side up.
  2 to 8 °C


General description

Milliflex® Cassette are ready to use agar-based media to be used with Milliflex® or Milliflex Oasis® system for convenient, rapid and reliable bioburden testing. The prefilled agar cassette helps to streamline the microbial contamination testing workflow with quality assurance and avoiding risks of exogenous contamination. Each plate is a 3-piece assembly containing approximately 10ml of the media. Heterotrophic Plate Count, formerly known as the standard plate, is a procedure for estimating the number of live heterotrophic bacteria in potable water. Peptic digest of animal tissue is the source of nutrients for organisms, which are not highly fastidious. Gelatin is utilized by microorganisms through a proteolytic mechanism and the addition of glycerol to the basal medium provides a source of carbon and energy.


Milliflex® Cassette prefilled with Heterotrophic Plate Count Agar is used in Bioburden testing and general microbiological analysis of water especially high purity water (electronics, RO, distilled or purified). It is widely used in pharmaceutical, cosmetic and brewing industry.


48 ea in 10 mL Agar in plastic Cassette

Culture Medium

Heterotrophic Plate Count

Analysis Note

Colonies appear clear to creamy white, some may produce pigment

QC organisms
E. coli (ATCC 25922)

P. aeruginosa (ATCC 9027)

S. aureus (ATCC 6538)

B. subtilis (ATCC 6633)

Target Microorganisms: Heterotrophic microorganisms

Legal Information

MILLIFLEX OASIS is a registered trademark of Merck KGaA, Darmstadt, Germany

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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