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NE1004 Sigma-Aldrich

Anti-Apolipoprotein E Mouse mAb (E6D7)

liquid, clone E6D7, Calbiochem®

Synonym: Anti-ApoE

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Properties

Related Categories AO-AQ, Alphabetical Index, Antibodies, Primary Antibodies
clone   E6D7, monoclonal
biological source   mouse
species reactivity   human
should not react with   mouse, rabbit
form   liquid
shipped in   wet ice
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
does not contain   preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   -20C

Description

General description

Anti-Apolipoprotein E, mouse monoclonal, clone E6D7, recognizes the ~35 kDa Apo E2, E3, and E4 in human serum and brain. It is validated for ELISA, WB, IP, and paraffin and frozen sections.

Mouse monoclonal antibody supplied as diluted culture supernatant. Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E.

Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum and brain.

Immunogen

Human

a synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of ApoE

Application




ELISA (see comments)

Immunoblotting (1:100-1:1000)

Immunoprecipitation (see comments)

Frozen Sections (1:100-1:10,000)

Paraffin Sections (1:100-1:10,000, formic acid or heat pre-treatment required)

Physical form

Culture supernatant diluted in PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Packaging

100 μL in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
Brain tissue, serum

Other Notes

Golabek, A.A., et al. 1996. J. Biol. Chem.271, 10602.

Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum, but multiple bands are seen when used with brain tissue samples. Staining of formalin-fixed paraffin-embedded tissues may be enhanced by the pre-treatment of tissue with 70% formic acid for 10-30 min at room temperature or by heat pre-treatment using citrate buffer, pH 6.0. This antibody has also been reported to work for immunoprecipitation and ELISA. Antibody should be titrated for optimal results in individual systems.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Peer-Reviewed Papers
15

References

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