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  • NE1004 - Anti-Apolipoprotein E Mouse mAb (E6D7)

NE1004 Sigma-Aldrich

Anti-Apolipoprotein E Mouse mAb (E6D7)

liquid, clone E6D7, Calbiochem®

Synonym: Anti-ApoE



Related Categories AO-AQ, Alphabetical Index, Antibodies, Primary Antibodies
clone   E6D7, monoclonal
biological source   mouse
species reactivity   human
should not react with   mouse, rabbit
form   liquid
shipped in   wet ice
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
does not contain   preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   -20C


General description

Anti-Apolipoprotein E, mouse monoclonal, clone E6D7, recognizes the ~35 kDa Apo E2, E3, and E4 in human serum and brain. It is validated for ELISA, WB, IP, and paraffin and frozen sections.

Mouse monoclonal antibody supplied as diluted culture supernatant. Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E.

Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum and brain.



a synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of ApoE


ELISA (see comments)

Immunoblotting (1:100-1:1000)

Immunoprecipitation (see comments)

Frozen Sections (1:100-1:10,000)

Paraffin Sections (1:100-1:10,000, formic acid or heat pre-treatment required)

Physical form

Culture supernatant diluted in PBS.


Following initial thaw, aliquot and freeze (-20°C).


100 μL in Plastic ampoule

Please refer to vial label for lot-specific concentration.


Toxicity: Standard Handling (A)

Analysis Note

Positive Control
Brain tissue, serum

Other Notes

Golabek, A.A., et al. 1996. J. Biol. Chem.271, 10602.

Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum, but multiple bands are seen when used with brain tissue samples. Staining of formalin-fixed paraffin-embedded tissues may be enhanced by the pre-treatment of tissue with 70% formic acid for 10-30 min at room temperature or by heat pre-treatment using citrate buffer, pH 6.0. This antibody has also been reported to work for immunoprecipitation and ELISA. Antibody should be titrated for optimal results in individual systems.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Antibody Basics

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