• USA Home
  • OP93 - Anti-BRCA1 (Ab-2) Mouse mAb (MS13)

EMAIL THIS PAGE TO A FRIEND
OP93 Sigma-Aldrich

Anti-BRCA1 (Ab-2) Mouse mAb (MS13)

liquid, clone MS13, Calbiochem®

Synonym: Anti-Breast Cancer Susceptibility Protein

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, BL-BZ, Primary Antibodies
clone   MS13, monoclonal
biological source   mouse
species reactivity   human
form   liquid
shipped in   wet ice
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
contains   ≤0.1% sodium azide as preservative
mfr. no.   Calbiochem®
storage condition   do not freeze
storage conditions   +2C to +8C

Description

General description

Anti-BRCA1 (Ab-2), mouse monoclonal, clone MS13, recognizes ~220 kDa BRCA1 in MCF-7 cells. It is validated for Western blotting, immunofluorescence, immunoprecipitation, and for paraffin sections.

Purified mouse monoclonal antibody generated by immunizing mice with the specified iummunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~220 kDa BRCA1 protein.

Recognizes the ~220 kDa BRCA1 protein in MCF-7 cells. Does not react with blood group antigens or growth factor receptors such as EGFR. Sold under license of U.S. Patent 5,753,441 and 6,162,897.

Immunogen

Epitope: within amino acids 1-304

Human

recombinant, human BRCA1

Application

Immunoblotting (2 µg/ml, chemiluminescence; see application references)

Immunofluorescence (see application references)

Immunoprecipitation (see application references)

Paraffin Sections (see application references)

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Legal Information

Sold under license of U.S. Patents 5,753,441 and 6,162,897.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

100 μg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
MCF7 cells

Other Notes

Antibody should be titrated for optimal results in individual systems.

Scully, R., et al. 1996. Science272, 123.
Gudas, J.M., et al. 1996. Cell Growth Differ.7, 717.
Vaughn, J.P., et al. 1996. Cell Growth Differ.7, 711.
Goldgar, D.E. and Reilly, P.R. 1995. Nat. Genet.11, 113.
Merajver, S.D., et al. 1995. Clin. Can. Res.1, 539.
Merajver, S.D., et al. 1995. Nat. Genet.9, 439.
Struewing, J.P., et al. 1995. Nat. Genet.11, 198.
Thompson, M.E., et al. 1995. Nat. Genet.9, 444.
Futreal, P.A., et al. 1994. Science266, 120.
Miki, Y., et al. 1994. Science266, 66.

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?