Assessing Enriched Murine CD4+ T Cells Differentiated Towards Effector T Helper Cell Lineages
Cellular differentiation, the process by which progenitor cells differentiate into specialized cell types, is fundamental to developmental biology. In the immune system, immune progenitor cells must ...
Keywords: Diseases, Flow cytometry, Gene expression, Growth factors, Inflammation, Microscopy, Transcription
Cell Viability and Proliferation Assays
Assays to measure cellular proliferation, cell viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper ch...
Keywords: Cancer, Cell disruption, Cell proliferation, Click chemistry, Enzyme-linked immunosorbent assay, Flow cytometry, Glycolysis, Immunocytochemistry, Immunohistochemistry, Indicators, Microscopy, Nucleic acid denaturation, Reductions, Solvents, Western blot
Counting Yeast Cells for Brewing and Wine Industries
During fermentation, carbohydrates present in the wort (a water-based solution of grain sugars) are converted by yeast into alcohol, carbon dioxide, and numerous byproducts. There are several stages ...
Keywords: Fermentation, Sample preparations, Size-exclusion chromatography
Handheld Cell Counter from Millipore
Scepter™ 2.0 is your portable cell counter. While other automated counters consume bench space and rely on object recognition software, manual focusing, and clumsy loading chambers, the Scepter™ cell...
Keywords: Cell culture, Environmental, Genomics
How to use Scepter™ 2.0 Cell Counter
The Scepter™ cell counter uses the Coulter principle of impedance-based particle detection to reliably and accurately count every cell in your sample.
Keywords: Cell culture, Infrared spectroscopy, Sample preparations
Human PBMC Isolation and Counting Using Scepter™ 2.0 Handheld Automated Cell Counter
Peripheral blood mononuclear cells (PBMCs) are blood cells with round nuclei, such as monocytes and lymphocytes, with the lymphocyte population comprised of T cells, B cells, and natural killer (NK) ...
Keywords: Anticoagulants, Centrifugation, Clinical, Flow cytometry, Gene expression, Purification, Separation
Immunomonitoring using the Scepter™ 2.0 Cell Counter and Scepter™ Software Pro
By: Amedeo Cappione, Ph.D.1; Emily Crossley2; Nagaraja Thirumalapura, DVM, Ph.D.3; and Debra Hoover, Ph.D.1
Keywords: Antibiotics, Cell culture, Cell proliferation, Centrifugation, Clinical, Environmental, Flow cytometry, Fractionation, Gene expression, Immobilization, Immunology, Indicators, Inflammation, Infrared spectroscopy, Microbiology, Sample preparations, Separation
Novel and Rapid Method to Assess Proliferative Properties of Mammalian Cells by Monitoring Cell Size Distributions
Much research has recently focused on elucidating the mechanisms by which eukaryotic cells regulate their size with respect to growth rate and cell cycle stage. These mechanisms are known to differ b...
Keywords: Apoptosis, Cell culture, Cell proliferation, Flow cytometry, High performance liquid chromatography, Sample preparations
Precise and Accurate Bead Counting Using the Scepter™ 2.0 Handheld Automated Cell Counter
Micron-sized beads are used in a variety of biological applications, ranging from daily validation of flow cytometer performance, to purification of fusion protein constructs from cell lysates. Depen...
Keywords: Immunoprecipitation, Purification, Sample preparations
Predicting Cell Death by Rapidly Assessing Size Changes with the Scepter™ Cell Counter
Apoptosis, the process of programmed cell death, is an essential component of many biological processes. Initial studies revealed that reduction in cell volume was an early morphological change durin...
Keywords: Apoptosis, Biological processes, Degradations, Flow cytometry, Microscopy, Reductions
Rapid Analysis of Human Adipose-Derived Stem Cells and 3T3-L1 Differentiation Towards Adipocytes
Obesity is a prevalent health risk in many industrialized nations, and its occurrence is closely associated with a number of pathological disorders, such as non-insulin dependent diabetes, hypertensi...
Keywords: Cancer, Cell culture, Diabetes, Microscopy, Obesity
Rapid Counting of Somatic Cells in Dairy Milk Using the Scepter™ 2.0 Cell Counter
Mastitis is an inflammatory change in the mammary gland most frequently characterized by pathological changes in mammary tissue. This potentially fatal infection is the most common and costly disease...
Amedeo Cappione, Ph.D. and Janet Smith
Keywords: Centrifugation, Clinical, Culture media, Detection methods, Flow cytometry, Microscopy, Sample preparations, Separation
The Scepter™ Cell Counter Performs with High Precision and Speed Across Multiple Cell Lines
Counting cells is often a necessary but tedious step for in vitro cell culture. Consistent cell concentrations ensure experimental reproducibility and accuracy. Cell counts are important for monitori...
Keywords: Cancer, Cell culture, Electronics, Gene expression, Transfection, transformation
Three-dimensional culture and assessment of drug-induced cell death using the CellASIC® ONIX Microﬂuidic Platform
Cultured cells have proved to be convenient, powerful model systems for elucidating biological processes and for testing the safety, effcacy and mechanism of therapeutic candidates. The main objectiv...
Keywords: Apoptosis, Biological processes, Cell culture, Centrifugation, Microscopy, Transcription
Alzheimer’s In A Dish™: 3D Neural Stem Cell Models of Alzheimer's Disease
Introduction Protocol • Human ReNcell® VM NSC Maintenance and Passaging • Viral Infection of Human ReNcell® VM NSCs • Enrichment of High-Expressing Transgenic Human ReNcell® VM NSCs • Cul...
Keywords: AGE, Alzheimer Disease, Apoptosis, Cell culture, Cell proliferation, Centrifugation, Deposition, Drug discovery, Flow cytometry, Gene expression, Immunohistochemistry, Microscopy, Optical microscopy, Transcription, Transduction, Western blot
Cancer Stem Cell Tumorsphere Formation Protocol
Solid tumors grow in a three-dimensional (3D) spatial conformation, resulting in a heterogeneous exposure to oxygen and nutrients as well as to other physical and chemical stresses. To mimic the 3D s...
Keywords: Cancer, Cell culture
Cell Analysis Best Practices [VIDEO]
How do you assess the state of your cell culture? We’ve got some best practices to share.
Keywords: Cell culture