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PHCC20060 EMD Millipore

Scepter 2.0 Handheld Automated Cell Counter

Rapid cell counts in a handheld easy to use format, includes pkg of 60 μm Scepter Cell Counter Sensors

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Properties

trade name   Scepter
application(s)   Cell Counting
particle size   6 µm–36 µm
cell size range   4.0–25.0 µm
material size   1 ea
shipped in   ambient

Description

Other Notes

The Scepter Cell Counter delivers rapid cell counts in a handheld easy to use format. Its on screen instructions guide you through the sampling process and in less than 40 seconds reveil data on cell concentration, average diameter, average volume and a histogram displaying population information on your culture. The device stores up to 72 counts which can be downloaded to your computer and uploaded to Excel for further manipulation.
FREE PACK OF 60μM Sensors included.

Background information

The Scepter 2.0 handheld automated cell counter provides a fast and convenient method for counting cells and particles and is compatible with both the 40 and 60uM sensor. The system employs the Coulter principle in a miniaturized, handheld, format enabling rapid cell counting; what used to take 10 minutes now takes less than 30 seconds. The user prepares a dilution of the cell culture of interest and uses the Scepter cell counter to aspirate a sample of this dilution into the Scepter sensor.

The Scepter cell counter’s screen displays:

Cell concentration
Average cell size
Average cell volume
Histogram of size or volume distribution
This system is intended for research use only and has been tested with cell types representative of those in use today.

Components

1 Scepter Cell Counter

1 Test Bead Vial

1 USB Cable

1 Downloadable Software

2 O-rings

50 Sensors (60µM)

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Assessing Enriched Murine CD4+ T Cells Differentiated Towards Effector T Helper Cell Lineages

Cellular differentiation is a fundamental process in developmental biology. Progenitor cells must have the ability to differentiate into more specialized cell types for the body to respond to infecti...
Keywords: Diseases, Flow cytometry, Gene expression, Growth factors, Microscopy, Transcription

Cell Counter Pipette from Millipore

Scepter™ 2.0 is your portable cell counter. While other automated counters consume bench space and rely on object recognition software, manual focusing, and clumsy loading chambers, the Scepter™ cell...
Keywords: Cell culture, Environmental, Genomics

Cell Viability and Proliferation Assays

Assays to measure cellular proliferation, cell viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. The proper ch...
Article Based on
BioFiles v6 n5, 17–21
Keywords: Cancer, Cell proliferation, Click chemistry, Enzyme-linked immunosorbent assay, Flow cytometry, Glycolysis, Immunocytochemistry, Immunohistochemistry, Microscopy, Nucleic acid denaturation, Reductions, Solvents, Western blot

Counting Yeast Cells for Brewing and Wine Industries

Yeast cells are critical to the fermentation process. During fermentation, carbohydrates present in the wort (a water-based solution of grain sugars) are converted by yeast into alcohol, carbon dioxi...
Keywords: Fermentation, Sample preparations, Size-exclusion chromatography

How to use Scepter™ 2.0 Cell Counter

The Scepter™ cell counter uses the Coulter principle of impedance-based particle detection to reliably and accurately count every cell in your sample.
Keywords: Cell culture, Infrared spectroscopy, Sample preparations

Human PBMC Isolation and Counting Using Scepter™ 2.0 Handheld Automated Cell Counter

Peripheral Blood Mononuclear Cells (PBMCs) are blood cells with round nuclei, such as monocytes and lymphocytes, with the lymphocyte population consisting of T cells, B cells, and natural killer (NK)...
Keywords: Anticoagulants, Centrifugation, Clinical, Coagulants, Gene expression, Purification, Separation

Immuno-monitoring using the Scepter™ 2.0 Cell Counter and Software Pro

Biological samples such as primary isolates or cultured cells are often heterogeneous mixtures of cells that differ by type and/or function. Such differences in cellular attributes are most commonly ...
Keywords: Antibiotics, Cell culture, Cell proliferation, Centrifugation, Clinical, Environmental, Flow cytometry, Fractionation, Gene expression, Immobilization, Indicators, Infrared spectroscopy, Separation

Novel and Rapid Method to Assess Proliferative Properties of Mammalian Cells by Monitoring Cell Size Distributions

Much research has recently focused on elucidating the mechanisms by which eukaryotic cells regulate their size with respect to growth rate and cell cycle stage. These mechanisms are known to differ b...
Keywords: Apoptosis, Cell culture, Cell proliferation, Flow cytometry, High performance liquid chromatography, Sample preparations

Precise and Accurate Bead Counting Using the Scepter™ 2.0 Handheld Automated Cell Counter

Micron-sized beads are used in a variety of biological applications, ranging from daily validation of flow cytometer performance to purification of fusion protein constructs from cell lysates. Dependi...
Keywords: Immunoprecipitation, Purification, Sample preparations

Rapid Analysis of Human Adipose-Derived Stem Cells and 3T3-L1 Differentiation Towards Adipocytes

Obesity is a prevalent health hazard in many industrialized nations, and its occurrence is closely associated with a number of pathological disorders, such as non-insulin dependent diabetes, hyperten...
Keywords: Cancer, Cell culture, Diabetes, Microscopy, Obesity

Rapid Assessment of Size Changes Using the Scepter™ Cell Counter can be Predictive of Cell Death

The process of programmed cell death or apoptosis is an essential component in many biological processes and is therefore the focus of much research. Initial studies revealed that reduction in cell v...
Keywords: Apoptosis, Biological processes, Flow cytometry, Reductions

Rapid Counting of Somatic Cells in Dairy Milk Using the Scepter™ 2.0 Cell Counter, Following Spin-Wash Sample Preparation

Mastitis is an inflammatory change in the mammary gland characterized by pathological changes in mammary tissue. This potentially fatal infection is the most common and most costly disease facing the...
Keywords: Centrifugation, Culture media, Flow cytometry, Microscopy, Sample preparations, Separation

The Scepter™ Cell Counter Performs with High Precision and Speed Across Multiple Cell Lines

Counting cells is often a necessary but tedious step for in vitro cell culture. Consistent cell concentrations ensure experimental reproducibility and accuracy. Cell counts are important for monitori...
Keywords: Cancer, Cell culture, Electronics, Gene expression, Transfection, transformation

Three-dimensional culture and assessment of drug-induced cell death using the CellASIC® ONIX Microfluidic Platform

Cultured cells have proved to be convenient, powerful model systems for elucidating biological processes and for testing the safety, effcacy and mechanism of therapeutic candidates. The main objectiv...
Keywords: Apoptosis, Biological processes, Cell culture, Centrifugation, Microscopy, Transcription

Protocols

3D Neural Stem Cell Models of Alzheimer’s Disease Protocol

Introduction Protocol    • Human ReNcell® VM NSC Maintenance and Passaging    • Viral Infection of Human ReNcell® VM NSCs    • Enrichment of High-Expressing Transgenic Human ReNcell® VM NSCs    • Cul...
Keywords: AGE, Alzheimer Disease, Apoptosis, Cell culture, Cell proliferation, Centrifugation, Deposition, Drug discovery, Flow cytometry, Gene expression, Immunohistochemistry, Microscopy, Optical microscopy, Transcription, Western blot

Cancer Stem Cell Tumorsphere Formation Protocol

Solid tumors grow in a three-dimensional (3D) spatial conformation, resulting in a heterogeneous exposure to oxygen and nutrients as well as to other physical and chemical stresses. To mimic the 3D s...
Keywords: Cancer, Cell culture

Related Content

Cell Analysis Best Practices [VIDEO]

How do you assess the state of your cell culture? We’ve got some best practices to share.  
Keywords: Cell culture

Peer-Reviewed Papers
15

References

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