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PHCC4 Millipore

Scepter Cell Counter Sensors

40 µm sensors designed to count particles between 3 µm & 17 µm

  •  eCl@ss 32011202

  •  NACRES NB.22

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Properties

Related Categories Labware, Manufacturer Browser, Millipore - Manufacturer Browser, Millipore Culture Supplies
Quality Level   200
mfr. no.   Scepter
parameter   50,000 particles/mL particle concentration (1,500,000 particles/mL)
application(s)   cell analysis: suitable (cell counting)
particle size   3-18 μm
compatibility   for use with cells sized 4.0–12.0 μm
shipped in   ambient

Description

General description

The Scepter 2.0 handheld automated cell counter provides a fast and convenient method for counting cells and particles and is compatible with both the 40 and 60uM sensor. The system employs the Coulter principle in a miniaturized, handheld, format enabling rapid cell counting; what used to take 10 minutes now takes less than 30 seconds. The user prepares a dilution of the cell culture of interest and uses the Scepter cell counter to aspirate a sample of this dilution into the Scepter sensor.

The Scepter cell counter’s screen displays:

Cell concentration
Average cell size
Average cell volume
Histogram of size or volume distribution
This system is intended for research use only and has been tested with cell types representative of those in use today.

The Scepter sensor is engineered with a micro-fabricated, cell-sensing zone that enables discrimination by cell size and cell volume - down to sub-micron and sub-picoliter resolution.

Coupled with precision liquid handling channels and electronics, the Scepter sensor accurately and reliably provides cell population statistics

Application

Research Category
Cell Culture

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Counting Yeast Cells for Brewing and Wine Industries

During fermentation, carbohydrates present in the wort (a water-based solution of grain sugars) are converted by yeast into alcohol, carbon dioxide, and numerous byproducts. There are several stages ...
Keywords: Fermentation, Sample preparations, Size-exclusion chromatography

Handheld Cell Counter from Millipore

Scepter™ 2.0 is your portable cell counter. While other automated counters consume bench space and rely on object recognition software, manual focusing, and clumsy loading chambers, the Scepter™ cell...
Keywords: Cell culture, Environmental, Genomics

How to use Scepter™ 2.0 Cell Counter

The Scepter™ cell counter uses the Coulter principle of impedance-based particle detection to reliably and accurately count every cell in your sample.
Keywords: Cell culture, Infrared spectroscopy, Sample preparations

Human PBMC Isolation and Counting Using Scepter™ 2.0 Handheld Automated Cell Counter

Peripheral blood mononuclear cells (PBMCs) are blood cells with round nuclei, such as monocytes and lymphocytes, with the lymphocyte population comprised of T cells, B cells, and natural killer (NK) ...
Keywords: Anticoagulants, Centrifugation, Clinical, Flow cytometry, Gene expression, Purification, Separation

Immunomonitoring using the Scepter™ 2.0 Cell Counter and Scepter™ Software Pro

By: Amedeo Cappione, Ph.D.1; Emily Crossley2; Nagaraja Thirumalapura, DVM, Ph.D.3; and Debra Hoover, Ph.D.1
Keywords: Antibiotics, Cell culture, Cell proliferation, Centrifugation, Clinical, Environmental, Flow cytometry, Fractionation, Gene expression, Immobilization, Immunology, Indicators, Inflammation, Infrared spectroscopy, Microbiology, Sample preparations, Separation

Precise and Accurate Bead Counting Using the Scepter™ 2.0 Handheld Automated Cell Counter

Micron-sized beads are used in a variety of biological applications, ranging from daily validation of flow cytometer performance, to purification of fusion protein constructs from cell lysates. Depen...
Keywords: Immunoprecipitation, Purification, Sample preparations

Predicting Cell Death by Rapidly Assessing Size Changes with the Scepter™ Cell Counter

Apoptosis, the process of programmed cell death, is an essential component of many biological processes. Initial studies revealed that reduction in cell volume was an early morphological change durin...
Keywords: Apoptosis, Biological processes, Degradations, Flow cytometry, Microscopy, Reductions

Rapid Analysis of Human Adipose-Derived Stem Cells and 3T3-L1 Differentiation Towards Adipocytes

Obesity is a prevalent health risk in many industrialized nations, and its occurrence is closely associated with a number of pathological disorders, such as non-insulin dependent diabetes, hypertensi...
Keywords: Cancer, Cell culture, Diabetes, Microscopy, Obesity

Rapid Counting of Somatic Cells in Dairy Milk Using the Scepter™ 2.0 Cell Counter

Mastitis is an inflammatory change in the mammary gland most frequently characterized by pathological changes in mammary tissue. This potentially fatal infection is the most common and costly disease...
Amedeo Cappione, Ph.D. and Janet Smith
MilliporeSigma
Keywords: Centrifugation, Clinical, Culture media, Detection methods, Flow cytometry, Microscopy, Sample preparations, Separation

The Scepter™ Cell Counter Performs with High Precision and Speed Across Multiple Cell Lines

Counting cells is often a necessary but tedious step for in vitro cell culture. Consistent cell concentrations ensure experimental reproducibility and accuracy. Cell counts are important for monitori...
Keywords: Cancer, Cell culture, Electronics, Gene expression, Transfection, transformation

Related Content

Cell Analysis Best Practices [VIDEO]

How do you assess the state of your cell culture? We’ve got some best practices to share.  
Keywords: Cell culture

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