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  • PIHA01250 - Millicell Cell Culture Insert, 12 mm, HA mixed cellulose esters, 0.45 µm

PIHA01250 Millipore

Millicell Cell Culture Insert, 12 mm, HA mixed cellulose esters, 0.45 µm

Mixed Cellulose Esters insert with pore size of 0.45 µm used in a 24-well plate for in vitro Toxicology Assays, Cell Attachment, cell culture, Cell Differentiation, Cell Surface Receptor Assays, Microbial Attachment Assays & Polarized Uptake Assays.

Synonym: cell culture inserts, permeable culture insert, tissue culture insert, tissue culture plate

  •  eCl@ss 32011202

  •  NACRES NB.22



Related Categories Cell Culture Supplies, Culture Plate Inserts, Labware, Manufacturer Browser, Millicell Standing Cell Culture Inserts,
Quality Level   400
material   mixed cellulose esters (MCE) membrane
sterility   sterile
packaging   pack of 48
mfr. no.   Millicell®
parameter   50 °C max. temp.
application(s)   cell analysis: suitable (cell attachment)
  cell analysis: suitable (cell culture)
  cell analysis: suitable (cell differentiation)
  cell analysis: suitable (cell surface receptor assays)
  cell analysis: suitable (polarized uptake assays)
  toxicology assay: suitable (in vitro)
H   10.5 mm
filter diam.   12 mm
filtration area   0.6 cm2
plate size   1 wells
matrix   MF-Millipore®
pore size   0.45 μm pore size
input   sample type: human embryonic stem cell(s)
sample type cardiac stem cell(s)
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type hematopoietic stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type neural stem cell(s)
surface area   0.6 cm2
Featured Industry   Industrial QC (microbial attachment assays)
shipped in   ambient


General description

Device Configuration: 24-well plate, 0.45μm pore size

With Millicell® inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.

Millicell® Standing Inserts:
- Promotes excellent cell growth and provides an exceptional opportunity for cell studies

Membrane Type:
MF-Millipore Membrane (mixed cellulose esters)
- For exceptional anatomical and functional polarization

Cell Attachment, Cell Growth, Cell Differentiation , Immunocytochemistry


50 pk in Individually blister packaged


Research Category
Cell Culture


Individually blister packaged

Legal Information

MF-Millipore is a registered trademark of Merck KGaA, Darmstadt, Germany

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


General Immunofluorescent Protocol for Millicell® Products

Immunocytochemical staining is a technique employing fluorescently labeled antibody, by which cells can be localized, labeled, and examined via fluorescent microscopy.
Keywords: Cell culture, Culture media, Fluorescent microscopy, Microscopy

Microscopic Examination of Samples

Millicell® devices using PET or CM membrane have been designed to allow visualization of cells from below using an inverted microscope. For viewing live cells, microscopic observations can be made th...
Keywords: Cell culture, Dehydration reaction, Immunofluorescence, Polymerization reactions, Positron Emission Tomography, Substitutions

Toluidine Blue Staining Protocol for Millicell

From our library of Articles, Sigma-Aldrich presents Toluidine Blue Staining Protocol for Millicell


Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

Note: Depending on the cell lines and the nature of the co-culture, the researcher can decide which side of the membrane is seeded first. To maintain sterile incubations of cells seeded on the unders...
Keywords: Cell culture

ECM Coating Protocols for Millicell

Coating of membranes and plastic surfaces with extra cellular matrices (ECMs) promotes cell attachment and monolayer formation. We have developed protocols for four types of ECMs on Millicell®-CM ins...
Keywords: Cell attachment, Cell culture, Condensations

Hematoxylin Staining for Millicell®-HA

Note: Standard histological hematoxylin and eosin staining techniques can be performed on thin sections.
Keywords: Cell culture

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

Note: This protocol is designed to grow undifferentiated embryonic stem cells in an indirect co culture with the fibroblast feeder layer. Although it is targeted for use with Millicell®-24 and Millic...
Keywords: Cell culture, Positron Emission Tomography

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