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PIHP03050 Millipore

Millicell Cell Culture Insert, 30 mm, polycarbonate, 0.4 µm

Polycarbonate cell culture insert with pore size of 0.4 µm used in a 6-well plate for cell attachment, cell culture, cell differentiation, drug transport & permeability.

Synonym: cell culture inserts, permeable culture insert, tissue culture insert, tissue culture plate

  •  eCl@ss 32011202

  •  NACRES NB.22

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Properties

Related Categories Cell Culture Supplies, Culture Plate Inserts, Labware, Manufacturer Browser, Millicell Standing Cell Culture Inserts,
Quality Level   400
material   polycarbonate membrane
  polystyrene
sterility   sterile
packaging   pack of 48
mfr. no.   Millicell®
parameter   50 °C max. temp.
application(s)   cell analysis: suitable (cell attachment)
  cell analysis: suitable (cell culture)
  cell analysis: suitable (cell differentiation)
  drug transporter assay: suitable
  permeability assay: suitable
H   13 mm
diam.   3.15 cm
filter diam.   30 mm
filtration area   4.2 cm2
plate size   1 wells
matrix   Isopore
pore size   0.4 μm pore size
input   sample type mesenchymal stem cell(s)
sample type pancreatic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type neural stem cell(s)
sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type cardiac stem cell(s)
surface area   4.2 cm2
shipped in   ambient

Description

General description

Device Configuration: 6-well plate, 0.4μm pore size

With Millicell® inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.

Millicell® Standing Inserts:
- Promotes excellent cell growth and provides an exceptional opportunity for cell studies
- Available with Biopore (PTFE) membrane, MF-Millipore (mixed cellulose esters) membrane, and polycarbonate membrane

Membrane Type:
Isopore Membrane (polycarbonate)
- For growth of attachment-dependent cells without matrix

Applications
Cell Attachment, Cell Growth, Cell Differentiation , Immunocytochemistry

Packaging

50 pk in Individually blister packaged

Application

Polycarbonate cell culture insert with pore size of 0.4 µm used in a 6-well plate for cell attachment, cell culture, cell differentiation, drug transport & permeability.

Research Category
Cell Culture

Packaging

Individually blister packaged

Legal Information

ISOPORE is a trademark of Merck KGaA, Darmstadt, Germany

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

General Immunofluorescent Protocol for Millicell® Products

Immunocytochemical staining is a technique employing fluorescently labeled antibody, by which cells can be localized, labeled, and examined via fluorescent microscopy.
Keywords: Cell culture, Culture media, Fluorescent microscopy, Microscopy

Microscopic Examination of Samples

Millicell® devices using PET or CM membrane have been designed to allow visualization of cells from below using an inverted microscope. For viewing live cells, microscopic observations can be made th...
Keywords: Cell culture, Dehydration reaction, Immunofluorescence, Polymerization reactions, Positron Emission Tomography, Substitutions

Toluidine Blue Staining Protocol for Millicell

From our library of Articles, Sigma-Aldrich presents Toluidine Blue Staining Protocol for Millicell

Protocols

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

Note: Depending on the cell lines and the nature of the co-culture, the researcher can decide which side of the membrane is seeded first. To maintain sterile incubations of cells seeded on the unders...
Keywords: Cell culture

ECM Coating Protocols for Millicell

Coating of membranes and plastic surfaces with extra cellular matrices (ECMs) promotes cell attachment and monolayer formation. We have developed protocols for four types of ECMs on Millicell®-CM ins...
Keywords: Cell attachment, Cell culture, Condensations

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

Note: This protocol is designed to grow undifferentiated embryonic stem cells in an indirect co culture with the fibroblast feeder layer. Although it is targeted for use with Millicell®-24 and Millic...
Keywords: Cell culture, Positron Emission Tomography

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